Lab 1 | Bradford Assay, Concentration

Molarity

mol/L, ng/mL, μg/mL, mg/mL

% - weight per vol (w/v)

Convert to decimal

Concentration measurements

Molarity, %, X

C₁V₁ = C₂V₂

Dilution formula

Dimensional analysis

Used to convert between different units of measurement

P20

1. Tens place

2. Ones place

3. Hundredths place

P200

1. Hundreds place

2. Tens place

3. Ones place

P1000

1. Thousands place

2. Hundreds place

3. Tens place

1:2 dilution

Add one part of a sample to an equal part of diluent, resulting in a solution with half the original concentration

Serial dilution

A technique that involves a stepwise series of dilutions of a substance, where each dilution is used to make the next one, resulting in a range of concentrations

Bradford Assay

Quantifies the protein concentration in a solution by measuring the color change of the dye Coomassie

Coomassie

Dye that binds to proteins, shifting from a red-brown to a blue color

Bovine serum albumin (BSA)

A protein found in cow blood and milk

Visible light spectrophotometer

A laboratory instrument that is capable of shining light of a specific wavelength on a sample held in a cuvette