Basic Biosafety Rules for Laboratory Personnel

Important Lab Safety Rules

Follow the instructions.

2. Dress appropriately.

3. Know the location of safety equipment.

4. Know what to do in case of an accident.

5. Don’t taste or sniff chemicals.

6. Don’t experiment on yourself.

7. Don’t eat or drink in the lab.

8. Don’t play mad scientist.

9. Leave experiments at the lab.

10. Dispose of waste properly

.hazard

Any source or condition that has the potential to cause injury, disease, harm or

damage is called

Risk

is the probability of the occurrence of the hazard and the degree of severity that

may occur when this possibility is realized

Risk type

Biological hazards chemical hazards physical hazards

(Possibility of exposure to hazards) ?

possibility of exposure during tests and operations)

individuals knowledge, experience, and safe working):

(Arrangement of activities to reduce likelihood of exposure

Purpose and Scope Gidesus*

.

The Biosafety program provides health and safety information to all employees and students who handle potentially hazardous biological materials (e.g., DNA, animals, and biohazardous agents) and assists in minimizing or eliminating associated risks.

The Biosafety Program serves the community by:

• .

:

• Providing information on safe procedures and practices.

• Explaining the proper use of safety equipment.

• Matching personal protective equipment to the task.

Pathological microbiology

Bactria virus fungi parasites and their toxins

Contamination can take place into forms

Directly and indirectly

Direct contamination,

Contamination without any intermediate between the source and infected microorgansing

Indirect transmission contamination

The transmission of infections agent though living and non living mediator that are contaminates by this factor

Aerosol

Aloud of liquid relased when a force is applied to the fluid and allowed to exit it

Way of transmission infectiousAgent in the lab

Commonly transmitted through inhalation or ingestion of aerosols, puncture of the skinordirect contact withskin through mucosal mays

The rules to prevent-such labs infections are listed bellow

Regular cleaning of surfaceS decontuming surfaces after spillage ordispersal, frequent washing of hands

What the most common cause infectionby ingestion

Pipetting by mouth and consuming food or drink inthelab

RG - 1 example

Bacillus subtitles& Escherichera Coli

RG2 example

Escherichia Coli , streptococcus pyogenes, candida albicans, herpes simplex virus type 1

RG -3 example

Shiga toksin

Escherichia coli

Mycobacterium tuberculosis

Shigella dysenteria tip 1

Echinococcus garmulosus s

RG - 4

Crimean-Congo hemorrhagic virus

Ebola virus

Lab coat selection guide

Flame re distant nomex, flame resistance treated cotton, standard cotton, fluid resistant

Flame resistant explain

Meta aramid synthetic fiber that provides the highest level fire protection. Open flames, electrical arc flash hazardous

Flame resistant treated cotton

Flame Resists nt, Treated Cottonia gian

Cotton lab coats that are treated with flame resistant material provide a good balance of protection from chemicals, and low-to-medium fire risk procedures.

• Appropriate for limited work with well-controlled open flames (e.g., bunsen burner)

• Appropriate for work with solvents and other flammable liquids

Standard cotton

100 % cotton are not as fire resistance

• Appropriate for use when working with chemicals, radioactive materials, or physical hazards, but only where risk of fire is low

• Flammable liquids - only appropriate for use with minor volumes of flammable materials

Fluid resistance lab Co et

100% polyester and prevent fluid pentratio n

• Appropriate for use when working with biological materials, bodily fluids, blood, blood-borne pathogens, tissue, cell lines, microbial cultures, and other potentially infectious materials.

Gloves type

Resistance heat, bas ) cold acid

Protection goggles and face shields

Googles, full safety glasses I face shield, UV protection glasses