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Bacillus on BA

Micrococcus on BA

Micrococcus on BA

E. coli on MAC
Klebsiella (mucoid)
Enterobacter

E. coli on EMB

Proteus

Klebsiella on EMB
Enterobacter

Klebsiella on MAC

K pneumoniae on EMB
mucoid

Streptococcus pneumoniae

Pseudomonas aeruginosa

Pseudomonas aeruginosa

Pseudomonas aeruginosa

Serratia

Serratia on BA

Serratia

Shigella

Shigella on HE
Providencia
Morganella

Shigella

Shigella on SS agar

Staphylococcus aureus on MSA

Staphylococcus species or Micrococcus on MSA

beta hemolysis GAS
(pyogenes)

beta hemolysis GBS
(agalactiae)

alpha hemolysis
Strep pneumo

alpha hemolysis
viridans

beta hemolysis

gamma hemolysis

alpha hemolysis

catalase +

catalase -

nonmotile

motile

Umbrella motility at room temperature
Listeria monocytogenes

bile esculin +

bile esculin -

Listeria monocytogenes, Bacillus species, Arcanobacterium haemolyticum
beta hemolytic GPB
Corynebacterium species, Bacillus anthracis
gamma hemolytic GPB
Erysipelothrix rhusiopathiae, Lactobacillus species
Alpha hemolytic GPB
positive

negative

positive

negative

positive

negative

susceptible

resistant

negative

positive

Neisseria gonorrhoeae

Neisseria meningitidis

Neisseria lactamica

Neisseria sicca

Moraxella catarrhalis

Neisseria gonorrhoeae

Neisseria meningitidis

Hemophilus parahaemolyticus

Hemophilus parainfluenzae

Hemophilus haemolyticus

Haemophilus influenzae

X factor
hemin is aka
V factor
NAD is aka
positive

negative

Haemophilus parainfluenzae and Haemophilus parahaemolyticus
have a positive porphyrin test
Strep pyogenes (GAS), Strep agalactiae (GBS)
Beta hemolytic Streptococci
Enterococcus, Strep agalactiae (GBS), Group D Strep (and Viridans Strep?)
Gamma hemolytic Streptococci
Strep pneumoniae, Viridans Strep, Enterococcus, Group D Strep
Alpha hemolytic streptococci
Susceptible

Resistant

positive

negative

resistant

Susceptible

positive

negative

positive

negative

positive

Negative

A/A. Ferments glucose, lactose, and/or sucrose. E. coli, Klebsiella, Enterobacter, Serratia, Yersinia.

A/A, H2S. Ferments glucose, lactose, and/or sucrose. H2S positive. P. vulgaris, Citrobacter.

K/A. Ferments glucose. Does not ferment lactose or sucrose. Shigella, Providencia, Morganella, P. shigelloides.

K/A, H2S. Ferments glucose. Does not ferment lactose or sucrose. H2S positive. Salmonella, P. mirabilis.

K/NC. Does not ferment glucose, lactose, or sucrose. Acinetobacter, Pseudomonas.

H2S positive, motility positive

indole positive, motility positive

indole negative, motility negative, H2S negative

positive

negative

-Alpha-naphthol (1st)
-40% KOH (2nd)
Voges Proskauer reagents
positive

negative

ferric chloride
Phenylalanine Deaminase reagent
positive

negative

Positive

Negative

Positive

Negative
