Pre and Post Lab Quiz 2

How should you check the inoculating tool is cool enough to collect a sample?

by lightly touching it to an uninoculated area on the agar plate

Which pipette would you use to measure/collect less than or equal to 1mL? 

micropipetter

Which pipette would you use to collect/measure volumes between 1mL - 50mL/

serological pipettes

How should you hold your pipette?

keep it straight up always

A micropipetter that is labeled with p200 or 20/200 has a max volume of ______?

200 uL

For a micropipetter you use the units ______ and for serological pipette your use the units ______ .

uL and ml

1000ul is equal to ___________ mL.

1

1mL of water is equal to _____ g

1

What type of paper should you use to clean the lens of the microscope?

lens paper

When do you need to use the immersion oil?

100x objective

How many streaks/quadrants should you have when completing a Streak for Isolation?

4

order of reagants for gram stain

1. crystal violet

2. iodine

3. decolorizing agent

4. safranin

A gram-negative culture will appear _________ when Gram-stained.

pink

A gram-positive culture will appear __________ when Gram-stained.

purple

All differential staining procedures have _________ key steps.

3

How do you prepare a smear?

Draw a circle on slide, Add bacterial sample using inoculating tool or pipette inside circle, wait for sample to dry

Cells that have thin peptidoglycan layers will be Gram-________________.

negative

Cells that have a thick peptidoglycan layer will be Gram-____________.

positive

How long must you wash your stain with acid alcohol?

10-15 seconds

Which stop must you go to when collecting samples?

first stop

What stop must you to release the sample?

second stop

The ability of lenses to show two adjacent objects a discrete entities is referred to as

resolution