Skin and Throat Normal Microbiota Lab

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Last updated 5:33 AM on 7/23/26
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30 Terms

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What is microbial antagonism?
Normal microbiota protect the body by competing with harmful microbes.
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Normal skin microbiota?
Propionibacterium, Staphylococcus spp., Micrococcus, Staphylococcus aureus.
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Staphylococcus characteristics?
Gram-positive cocci, catalase-positive.
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Catalase test detects?
Catalase enzyme.
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Positive catalase test?
Bubbles form.
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Coagulase-positive bacterium?
Staphylococcus aureus.
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Coagulase test detects?
Coagulase enzyme.
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Positive coagulase test?
Plasma clots.
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Purpose of Mannitol Salt Agar (MSA)?
Selects for Staphylococcus and differentiates mannitol fermentation.
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Why is MSA selective?
High salt inhibits most bacteria except Staphylococcus.
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Yellow MSA means?
Mannitol fermented.
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Red/Pink MSA means?
No mannitol fermentation.
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Materials for Exercise 4-4?
MSA plate, sterile swab, sterile saline.
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First step of Exercise 4-4?
Wet swab with saline and swab skin.
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How is MSA inoculated?
Swab half the plate and streak for isolation.
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What colony is subcultured?
Catalase-positive, Gram-positive cocci.
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Normal throat microbiota?
Staphylococcus, Neisseria, Haemophilus, Streptococcus.
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Beta-hemolytic Streptococcus?
Streptococcus pyogenes.
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Alpha hemolysis?
Partial RBC destruction; green zone.
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Beta hemolysis?
Complete RBC destruction; clear zone.
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Gamma hemolysis?
No hemolysis; no change.
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Purpose of Blood Agar?
Detect hemolysis.
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Materials for Exercise 5-25?
Blood agar plate.
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Blood Agar procedure?
Swab half the plate and streak for isolation.
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Three hemolysis types?
Alpha, Beta, Gamma.
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Which test identifies catalase?
Catalase test.
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Which test identifies S. aureus?
Coagulase test.
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Which bacterium is catalase- and coagulase-positive?
Staphylococcus aureus.
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Which medium tests mannitol fermentation?
Mannitol Salt Agar.
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Which medium shows hemolysis?
Blood Agar.