BIOL 458 (Microbio) Lab Exam 1

What PPE must be worn in lab?

• Lab coat

• Gloves when working with microorganisms

What length must shorts/skirts/pants be?

Under the knee

What is the correct order of clean-up when coming in and leaving?

1) Wash hands

2) Spray bench top with 70% ethanol

3) Do experiment

4) Spay bench top with 70% ethanol

5) Wash hands

Where is paper disposed?

Recycling

Where are paper towels disposed?

Trash can

Where are gloves disposed?

Biohazard bins

Where is broken glass disposed?

Broken glass container (with other sharps)

True or false: liquid cultures need to be autoclaved prior to disposal

True

Where are agar plates/solid wastes disposed?

Biohazard waste

Define BSL1 and give an example

Organisms do not typically cause disease in healthy individuals, ex. E. coli

Define BSL 2 and give an example

A moderate risk of infection, primarily in immunocompromised individuals, ex. salmonella

Define BSL3 and give an example

Infects healthy people, low infectious dose, deadly, ex. TB

Define BSL4 and give an example

Deadly viruses, hemorrhagic diseases, ex. ebola

Which BSL levels do we use in lab?

1 and 2