ocr biology food test with the reason and hazards
what do you uses to test for sugar ?
Benedict's test is used to detect sugars
Explanation of Benedict solution ?
Reducing sugars react with Benedict's solution, like glucose, producing a red-brown precipitate. The color may vary depending on the presence of glucose, with green, yellow, or orange being more common.
what are the hazards/saftey call of Benedict solution ?
Wear safety goggles.
Benedict's solution is an irritant.
Avoid contact with skin and eyes.
Take care when heating in a boiling water bath.
what do you uses to test for starch ?
Add iodine solution to the food being tested.
Explanation of iodinne solution ?
Foods containing starch will turn a blue-black colour.
The iodine test can also be used with a microscope to stain starch grains in plant cells.
what are the hazards/saftey call of iondine solution ?
Wear safety goggles.
Iodine solution is an irritant.
Avoid contact with skin and eyes.
what do you uses to test for protiens ?
The biuret test is used to detect peptide bonds in proteins.
Explanation of iodinne ?
Add Biuret solution A to a solution of the food being tested and mix carefully. Then trickle a little Biuret solution B down the side of the tube. Look for a purple colouration where the solutions meet.
Biuret reagent is sometimes available as a single solution.
what are the hazards/saftey call of Biuret solution ?
Wear safety goggles.
Biuret solution A is corrosive.
Biuret solution B is an irritant.
Avoid contact with skin and eyes.
what do you uses to test for fats ?
The Sudan III test is used to test detect lipids
Explanation of Sunda III ?
One common test for the presence of lipids is the Sudan III test, which involves adding a small amount of Sudan III stain to the unknown solution and observing for a red color change. hence will inform of the presence of lipids.
what are the hazards/saftey call of Sunda III solution ?
Wear safety goggles.
Sudan III solution is flammable as it is dissolved in alcohol.
Avoid contact with skin and eyes.
what do you uses to test for lipids ?
The emulsion test is an alternative test for lipids. using Ethonal and water.
Explanation of ethonal and water ?
To test for the presence of lipids, you would carry out the emulsion test. To do this, you take the sample and mix it with equal volumes of ethanol and water followed by shaking. A cloudy white emulsion will form if lipids are present. If lipids are absent, no emulsion will form.
what are the hazards/saftey call of ethonal and water ?
Wear safety goggles.
Ethanol is flammable.
Avoid contact with skin and eyes.