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LIGHT MICROSCOPE
most common
different absorption of light that is bettered by staining
light passes and that is what we see
PHASE CONTRAST MICROSCOPE
optical device that can show minute distances (details)
INTERFERANCE MICROSCOPE
two beams of light and can measure distance of tissues
FLUORESCENT MICROSCOPE
uses uv (different wavelengths) and give you different colors
TRANSMISSION ELECTRON MICROSCOPE
does NOT use light, uses beam of electrons that are bombarded on the slide
cannot see with the naked eye ( can hurt your eyes)
SCANNING ELECTRON MICROSCOPE
can use tv monitor to move to screen for specimen separation
specimen prep for electron microscopy
must be dehydrated (NO WATER)
Fixative
chemical (osmium tetroxide)
with both electrons microscope you will
be given a picture with no color (color later added)
atomic force microscopy
most powerful
can literally feel the surface of cell with tool (fingertip probe)
probe passes surface