PTC Genotyping/DNA Fingerprinting

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7 Terms

1
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reagents used in PTC extraction

  • Detergent

    • Tris

    • Protease

  • Salt (NaCl)

  • Isopropyl Alcohol (ethanol)

2
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Detergent function

breaking the cell open

  • Tris: maintain pH

  • Protease: digests proteins and removes them from histones

3
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Forward primer

  • Identical to coding strand

  • Anneals to complementary strand

  • 5’ to 3’

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Reverse primer

  • Identical to complementary strand

  • Anneals to coding strand

  • 3’-5’ → 5’-3’ (reverse complement) when read

5
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DNA ladder (molecular weight ladder)

  • use as standard samples

  • size reference

6
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salt (NaCl) function

causes DNA to form a precipitate

7
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Isopropyl Alcohol (ethanol)

cause DNA to clump together