DNA extraction lab quiz

Why do we "crush" the strawberry fruit during DNA extraction?

Crushing the strawberry fruit physically breaks apart the cell walls

Why do we use dish soap in the DNA extraction process?

Dish soap disrupts cell and nuclear membranes by dissolving lipids and proteins, helping to release the DNA.

What is the role of salt in DNA extraction?

Salt neutralizes the negative charges on DNA so strands stick together, and it causes proteins and carbohydrates to precipitate.

What does cold ethanol do in DNA extraction?

It pulls water from the DNA so it collapses and precipitates out, becoming visible as white, mucous-like strands.

Why don't we use room temperature ethanol?

Cold ethanol increases the amount of DNA that precipitates compared to room temperature ethanol.

In a plant like a strawberry, what must DNA be isolated from during extraction?

From all other cell parts: cell wall, membranes, mitochondria, vacuoles, ER, Golgi apparatus, lysosomes, etc.

What steps are used to extract DNA from strawberries?

1.Physically crush the fruit. 2. Use detergent to break membranes. 3. Filter the mixture. 4. Add ethanol to precipitate the DNA.

What are some uses for extracted DNA?

Forensics, paternity testing, genetic screening, crop modification, and transferring genes between organisms for specific traits.