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Produce VFA (Cleave B1-4glycosidic linkage)
Synthesisze Vit B
Produce vital proteins for host
What are benefits of the rumen microbiome?
Buffers the acids generated by fermentation,
Provides phosphate for ATP
Provides Nitrogen for protein synthesis
What does a ruminants saliva do for the rumen microbiome?
Acetic 60% (milkfat)
Propionic 20% (glucogenic)
Butyric acid 15% (bacterial)
What 3 VFA does cellulose produce when digested by the rumen?
More propionate
What VFA do high concentration rations produce when digested in thr rumen?
Flora activity (is animal anorexic?)
Flora imbalances (grain overload?)
Subclinical acidosis
Intoxications
What is ruminal fluid analysis used for?
Fenestrated pipe placed into rumen to the bottom, and oro-ruminal tube passed through pipe and cyphoned into bucket
How is rumen fluid collected from a fistualted cow?
Frick speculum protects tube, head facing down and tub advanced 200ml aspirated
How is rumen fluid collected from a oro-ruminal tube?
Alkaline saliva can increase pH reading
What can a too small sample of ruminal fluid collected via oro-ruminal tube cause?
4-6" 14-16G needle advanced into the ventral sac of rumen aseptically
WHow is rumen fluid collected from a ruminocentesis?
Avoids salivary contamination
Poss contamination to peritoneal cavity
What are benefits of a ruminocentesis? Cons?
Forage: Olive green/ brown - rich odor
Grain/silage: Tan/green
Acidosis: yellow/grey - sour odor
What color and odor of ruminal fluid would you expect of the following diets?
Forage:
Grain/silage:
Acidosis:
Lower
Starches and sugars as well as finly chopped feeds tend to ___ the pH?
Grain -> 2-4hrs
TMR ->4-8hrs
When is the pH the lowest after a grain meal? TMR?
4-6hrs post death
When is the rumen pH no longer viable in a cadaver?
6.2-7.0
What is the pH range for a forage diet?
5.7-6.5
What is the pH range for a grain diet?
-
+
In a forage diet gram ____ will be the primary type of bacteria in rumen fluid, in grain diet/ overload gram ___ will be the primary type?
Holotrichs - large
Entodiniomorphs - small
In an acidois ____ protizoa will die first and at a pH of 5.7 _____ will die off?
Insufficent nutrients
Acidosis
Toxins
Name 3 reasons why you may have inacctive or absent protizoa in fresh ruminal fluid?
Meausres Redox potentia/ metabolic capacity (normal if blue disapears b/4 6 mins)
What does the methylene blue reduction test do?
10-25mEq/l
What is normal rumin fluid Cl- concentration?
Internal vomiting - HCl into rumen
Why do we check rumen chloride concentrations?
Abomasal displacement
Pyloric obstruction
What can cause internal vomiting?
Dramatic fall in milk production
Cranial abd pain - taut abd, refusal to turn sharp corners
What are clinical signs of Tramatic reticuloperitonitis?
Muffled heart sounds
Pericardial splash
Pleural fluid w/o pericardial fluid - radiating heartbeat
Edema of the brisket (R-sided heart failure)
What are some PE findings of Tramatic reticulopericarditis?
U/S
Abdominocentesis -> if reticular abscess (high-protein fluid) low protozoa activity
How is traumatic reticuloperitonitis DX?
High L flank laparotomy W/ distal paravertebral analgesia
- prevent leakage of rumen into peritoneum
How is traumatic reticuloperitonitis treated?
Magnet
Tulathromycin - extra label -> monitor withdraw from milk products
oral fluids
Flunixin meglumine
Ruminal fluid transfusion
What is medical managment of TRP if O declines sx?
SLOW - up to 4w for milk production ot improve
ABX tx alone likely wont improve
What is recovery for traumatic reticuloperitonitis?
Liver abscess
Endocarditis
Chronic suppurative pneumonia
Pleural abscess
What are 4 other things on your Ddx if you are suspecting if there is omasum or diaphragm involvement w/ traumatic reticuloperitonitis?
T. pyogenes
What is the most common bacteria to form abscesses in cattle w/ TRP
Magnets prophylacitcally
What is used to contorl traumatic reticuloperitonitis?
1. Esophageal obstructions
2. Swapped from forage to high grains
What are two common causes of free gas bloat?
Alfalfa
Clovers
Wheat
What types of legums often cause frothy bloat?
Vagal defects
Hardware dz/ obstruction
Post-weaning issues -> lack of rumen development
What are possible causes of chronic free gas bloat?
Abd distension - L paralumbar fossa
Gasping
Tail raised (position for Urination/ defication)
What are clinical signs of bloat?
Extremities/ upper esophagus - hemorrhagic
Rumen/abd/ lower esophagus - pale
Prolappsed rectum/ vagina
What are the typical findings on necropsy w/ bloat?
Stomach tub - can theraputic
How is free gas bloat dx?
Rumenotomoy/ troncarizaton
Stomach tube - poloxalne -> detergent/ surfactant
How is frothy bloat tx?
Temporary fistula - 4-6 w to heal
How is chronic bloat tx?
Poloxalene
Dry roughage/ reduced intake
abx (Pen/ tetracyclinesa) -> resistance quick
How is bloat prevented?