chemical basis of histological staining

how do they gain these certain colors?

1. due to the reactions

2. proteins have special properties that produce these colors

what is the meaning of amphoteric properties of proteins?

proteins will behave differently under basic and acidic mediums

why do they assume different colors?

because they behave diff under basic and acidic mediums

isoelectric point

pH where molecules have NO NET ELECTRICAL CHARGE (aka neutral)

ph

negative log of H+ concentration

both acids and bases

are proton donors (h+) and acceptors

acidophilic

macromolecules attracred to acidic dye

basophilic

macromolecules attracted to basic dyes

dna is an acid

so attracted to a basic dye

basic dye attracted to

negative charge

acidic dyes attracted to

components that carry positive charges