Micro CLS Quiz 1

Truants Stain

acid fast stain

acid fast stain

Auramine-rhodamine fluorochrome

what do bacteria need

• carbon

• Nitrogen

• ATP

5% sheeps blood agar

Nutritional with sheeps blood

Chocolate agar

Nutritional with NAD ad sheeps blood

SBA with colistin and Naladixic acid (CNA)

selective with naladixic acid and Colistin

MacConkey

Selective and differential with lactose, Crystal Violet, Bile salts

MACSORB

selective and differential with Sorbitol, crystal violet and bile salts

XLD

Selective and slightly differential with Xyrose, Lysine, LActose, Sucrose, Sodium deoxylate, sodium thiosulfate

XLD is mostly used for what

Stool cultures

Ex. Shigella and salmonella

Macsorb is mostly used for

isolation of E.coli

CVA

selective with brucella basal media and antibiotics

MTM

selective with Colistin, trimethylprim, vancomyocin, and nystatin

Crystal violet in a gram stain is what type of stain

Primary

Gram’s Iodine is used for what in a gram stain

mordent

acetone and alcohol in a gram stain is used for what

decolorizer

safranin in a gram stain is used for what

counter stain

Capnophilic

high CO2

facultative

can switch between O2 and CO2

Microaerophilic

minimal oxygen loving

Other Lab Results
Associated with Infection

Increased sedimentation rate

Peripheral leukocytosis

Increased gamma-globbulins

Sterilization

a validated process used to render a product
free of all forms of viable microorganisms,
including all bacterial endospores

Decontamination

reduction of pathogenic microorganisms to a
level where items are safe to handle without
protective attire

Disinfection

elimination of most, if not all, pathogenic
microorganisms excluding spores
used on inanimate objects

Antisepsis

disinfection of living tissue

What type of specimen would routinely be inoculated onto XLD agar?

stool

Which is the lowest class of biosafety cabinet that will offer protection to both the laboratory worker and the clinical material within the cabinet?

Class II

What biosafety level is necessary for working with agents associated with human disease, but generally not transmitted via aerosols?

BSL-2

Which of the following set of ingredients are found in modified Thayer- Martin agar?

a. Colistin and nalidixic acid

b. Vanocmycin, colistin, trimethoprim and Nystatin

c. Vancomycin, cefoperazone, and Amphotericin B

d. Crystal violet and bile Salts

b. Vanocmycin, colistin, trimethoprim and Nystatin

Which of the following best represents a microaerophilic environment?

a. 5-6% O2

b. 5-10% CO2

c. 0% O2

d. 21% O2 and 1% CO2

A. 5-6% O2

A gram stained smear was prepared from a 18hrs old culture of E.coli. On microscopic exam, the organism appeared blue. These organisms were:

under decolorized

A gram stained smear was prepared from a colony of staph. aureus. the reagents were used in the following order:

1. Crystal Violet

2. water rinse

3. acetone/alcohol decolorizer

4. water rinse

5. safranin

6. water rinse

The expected color of these organisms is:

Red

A mixture of E. coli and staph. aureus is inoculated onto blood agar, CNA agar, and MacConkey agar and incubated overnight at 35 degrees. The expected growth pattern is:

CNA: S. aureus

MacConkey: E. coli

Blood agar: S. aureus and E.coli

An organism was subcultured to 4 blood agar plates. When the plates were incubated at 35 degrees under different atmospheric condition, the following results were observed:

• aerobic: growth

• Anarobic: growth

• 5% CO2: growth

• Microaerophilic: growth

  the organism is best described as:

Facultatively anaerobic

which of the following lab results would correspond with a patient that has an infection?

a. increased sedimentation rate

b. decreased sedimentation rate

c. peripheral leukocytosis

d. Both a and c

e. Both B and C

D. both A and C

The label on the bottle of commercially prepared chemical solution states that the solution should be used to decontaminate work benches and that it does not kill spores. This solution is an example of

a disinfectant

The Co2 content in incubators is checked by using a

Fyrite device