week 10 lab

pink/red

what color would show for a positive result with the litmus cream

blue/purple

what color would show for a negative result with the litmus cream

with more digestion (lipids being broken down into fatty acids by the enzyme lipase) the more acidic it becomes, which lowers the pH

more H+ released

explain the pH relationship with the litmus cream as lipids are digested

pH stays basic (high pH) with the absence of fatty acids from no digestion

less H+ released

explain the pH relationship with the litmus cream as lipids aren’t digested

pink

is the positive control with lipids pink or purple/blue

purple/blue

is the negative control with lipids pink or purple/blue

orange

in the positive control of the iodine test for carbohydrates is it orange or black

black

in the negative control of the iodine test for carbohydrates is it orange or black

orange

what color is a positive result with carbohydrates in the iodine test

black

what color is a negative result with carbohydrates in the iodine test

too high of temperature denatures the enzyme and results in negative result

too low of temperature slows the enzymes activity causing there to be little to no reaction

what is the relationship between temperature and enzyme activity

positive result in litmus test - lipids digested into fatty acids causing a lower pH (more acidic because hydrogen ions are being released)

what does a pink result mean

negative result in litmus test- lipids not digested into fatty acids causing a higher pH (stays basic because no hydrogen ions are being released)

what does a purple/blue result mean

starch was broken digested into sugars, iodine not able to bind to starch due to its absence

what does an orange/yellow result mean for iodine test

starch was not digested into sugars, iodine binds to starch due to its absence

what does an black result mean for iodine test

starch was not digested into sugars, and benedicts solution couldn’t find sugar

what does a blue result for benedicts test mean

starch was digested into sugars and the benedicts solution was able to find sugars

what does an orange/red result for benedicts test mean

benedicts solution is only able to find some sugar and not all because some starch still needs to be digested into sugars

happens at 0 degrees

what does a blue/green result in benedicts test mean

proteins weren’t digested into amino acids by trypsin

what does a clear result mean for protein digestion

proteins were able to be digested into amino acids by trypsin

what does a yellow result mean for protein digestion

not all of protein was able to be digested into amino acids by enzyme, its done partial digestion

what does a clear/yellow tint mean for protein digestion

if pH is too acidic or too basic the enzyme will denature or work too slowly, the pH needs to be neutral

but in the stomach it prefers acidic for stomach acid

in the intestine it prefers it basic/neutral

explain the relationship of pH in protein digestion

true

t/f: if pH is too acidic or too basic the enzyme will denature or work too slowly, the pH needs to be neutral for all lipids, proteins and carbs