5-Gene-expression analysis by Micro arrays, digita; PCR-05-08-2024

Microarray

A technology used to analyze the expression levels of thousands of preselected genes in one experiment through nucleic acid hybridization.

Hybridization

The process of binding complementary DNA fragments to probes on a microarray.

Oligonucleotides

Short sequences of nucleotides used as probes in microarrays.

Fluorescent Detection

Methods used to visualize hybridized samples on microarrays using fluorescent dyes.

cDNA

Complementary DNA synthesized from mRNA, used in microarray experiments.

SNP (Single Nucleotide Polymorphism)

A variation at a single position in a DNA sequence among individuals.

RNA-Seq

A high-throughput sequencing technique used to analyze the transcriptome of a cell.

Poly-A Selection

A method used to enrich for mRNA by selecting RNA molecules with poly-A tails.

Digital PCR

A nucleic acid detection and quantification method that partitions a sample into numerous parallel PCR reactions.

Affymetrix Technology

A method for producing DNA microarrays where DNA sequences are synthesized directly on the surface.

Ribo-depletion

A protocol that removes ribosomal RNAs to enrich the sample for other RNA types.

Massive Parallel Signature Sequencing (MPSS)

A technique for sequencing transcripts to provide insights into gene expression levels.

Hybrid capture

A method for selectively enriching specific regions of interest in RNA samples.

SAGE (Serial Analysis of Gene Expression)

A technique that generates cDNA fragments from mRNA to analyze gene expression.

Dynamic Range

The range of expression levels that can be accurately detected by a method.

Microarray Types

Includes spotted arrays, self-assembled arrays, and in-situ synthesized arrays.

Confocal Scanner

A device used to detect and generate digital images from hybridized microarrays.

Droplet Digital PCR (ddPCR)

A digital PCR method that partitions samples into droplets for improved accuracy and sensitivity.

Absolute Quantification

Counting the exact number of targets in a sample without the need for a standard curve.

Pathogen Detection

Using microarrays to identify the presence of infectious agents in a sample.