Biology Microscope Lab

Microscope

Laboratory Instrument used to examine objects that are too small to be seen by the naked eye

Optical Microscope

Uses lenses to refract visible light that passed through thinly sectioned sample to refract light reflected from the surface of an object to produce an observable image

Major Microscopes

Fluoresence Microscope, Electron Microscope, and Scanning Probe Microscope

Microscopy

The Science of investigating small objects and structures using a microscope

Microscopic

Being invisible to the eye unless aided by a microscope

Robert Hooke

Curator of Experiments of Royal Society of London whom created Micrographia and coined the term “cells” when discovering plant cells in a slice of cork

Virtuoso

Able to contribute findings of major importance in any field of science

Micrographia

Book created by Robert Hooke that contained detailed record of observations from magnified drawings of studied organisms

Antonie Van Leeuwenhoek

Dutch microbiologist & Microscopist known as father of microscopy as first to observe and experiment with microbes using self-made single lense microscope & publishes letters in Royal Society’s Philosophical transactions

Magnify

To make something appear larger than the original size

Resolve

The ability of an optical instrument to distinguish 2 closely spaced objects as separate entities

Parfocal

Different objective lenses or eyepieces share same focal plane

Total magnifying power

is the degree to which an optical system enlarges an object, calculated by multiplying the magnifications of the objective and eyepiece lenses.

TMP

Objective lense power x Ocular lense power

Transmission Electron Microscope

Microscopy technique where a beam of electrons is transmitted through an ultra-thin speciment

Scanning Electron Microscope

Instrument that generates highly modified 3D images of sample surfaces by scanning them with focused beam of electrons

Compound Light Microscope

Optical Instrument that uses visible light and a system of multiple lenses to magnify specimens.

FOV

Field of view

The 4 Objective Lenses

Scanning Power Objective(4x), Low Power Objective(100x), High Power Objective(400x), Oil Immersion Objective(1000x)

Oil Immersion Objective

Lens designed to be used with drop of immersion oil between lens & specimen, emulating opacity & refractory properties of glass in lens and slide so light doesn’t bend and cause blurred image

Steps for using a microscope

1. Clean all objective & Ocular Lenses

2. Observe scope for damage

3. Check that light bulb & dimmer are set-up and work

4. Make sure objective lenses are set to initial scanning power objective(4x)

5. have ocular lense at 10x and scanning power in conjunction

6. Increase microscope magnification by switching “up” in power to higher objective lens use - rotate nose piece to next magnification objective

7. Consider reversal in all observations - left is right, up is down

8. Clean all lenses when finished

Light Microscope Cell Imagery

TEM(Transmission Electron Microscope) Imagery

SEM(Scanning Electron Microscope) Imagery

Compound Microscope

Parts of the Compound Microscope