Lecture 5 - Parasitic Bacteria

Why is Chlamydiaceae an obligate intracellular?

deficient in energy metabolism

Which morphology is in the infectious stage?

elementary body

Which morphology is the multiplying stage?

reticulate body

Which organisms are released from the cell through reverse endocytosis?

C. pneumoniae & C. trachomatis

Which organism is released from the cell through cell lysis?

C. psittaci

Which organism is detected through direct fluorescence assay?

C. trachomatis

What is the drug of choice for parasitic bacteria?

tetracycline or fluoroquinolones

Why is tetracycline or fluoroquinolones the drugs of choice?

don’t target cell wall synth

Which organism is a facultative anaerobe?

Mycoplasma

Which organism is fastidious?

Mycoplasma

What provides structure to the cell if there is no cell wall?

sterols

Which organism lives on the cell surface?

Mycoplasma

Which organism has fried egg morphology?

M. pneumoniae

What media is used for M. pneumoniae?

modif NYC or PPLO

Which organism causes atypical/walking pneumonia?

M. pneumoniae

Which organisms have an elementary body & reticulate body?

Chlamydiaceae & Ehrlichia

Which Chlamydiaceae sp causes the STI?

C. trachomatis

What media is used for C. trachomatis?

McCoy

Which organisms can be visualized with Giemsa stain?

C. trachomatis & Ehrlichia

Where does PID replicate?

mononuclear phagocytes

What media is used for C. pneumoniae?

HL or Hep-2 cells

Which organism is spread by birds?

C. psittaci

Which organism is prevelent in the SE Atlantic and south & central states?

R. rickettsii

Which organisms are spread by ticks?

R. rickettsii & Ehrlichia

Which organism causes rocky mtn spotted fever?

R. rickettsii

Which organism is spread by louse?

R. prowazekii

Which organism is spread in unsanitary conditions like war, famine, and natural disasters?

R. prowazekii

Which organism causes epidemic typhus?

R. prowazekii

Which organisms are visualized using IFA?

R. prowazekii & R. typhi

Which organism is spread through rat/cat fleas?

R. typhi

Which organism causes endemic typhus?

R. typhi

Which organism is prevalent in Japan, Australia, S Pacific Islands?

Orientia tsutsugamushi

Which organism is spread by mites (chiggers)?

Orientia tsutsugamushi

Which organism causes scrub typhus?

Orientia tsutsugamushi

Which organism is prevalent worldwide except New Zealand?

Coxiella

Which organism causes Q fever?

Coxiella

Where does Coxiella replicate?

lysosomes

Which organism is stable extracellularly?

Coxiella

Which organism exhibits morulae?

Ehrlichia

What is morulae?

cluster of bacteria in phagosomes

What stains are used for Ehrlichia

Giemsa & Wright

Which organism causes HME?

Ehrlichia

Where does Ehrlichia replicate?

phagosomes of monocytes