BIOC2306 Enzyme kinetics 2 unfinished

what is surface plasmon resonance

technique that tells us the mass of something that is bound to a surface (so can bind enzyme to surface and flow sub over see if binds)

uses a lazar and angle of diffraction which changes based on mass of bound sub

what equipment is used for surface plasmon resonance

plug flow reactor

how can surface plasmon resonance be used

to test which substrate binds first to determine mechanism

what is equilibrium dialysis

method to determine which substrate binds first, performed separately for each substrate. membrane in solution only containin enzyme but perm to sub, inouter solution. Req reach equilibrium, if enzyme binds sub then more sub in membrane as due to eq (ES and S free greater than just S free on outside)

what are the limitations of equilibrium dialysis

req to reach equilibrium therefore cant perform with unstable complex

what can product inhibition patterns be used for

to