BIOC 511 Exam 1: Eukaryotic Replication Players and Funcitons

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Last updated 11:11 PM on 9/22/26
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7 Terms

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DNA pol alpha

priming during replication

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DNA pol gamma

mitochondrial DNA replication

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dna pol delta

main replicative enzyme

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dna pol kappa

required for attachment of cohesin proteins which hold sister chromatids together until anaphase stage of nuclear division

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Helicase (MCM complex)

eukaryotic clamp loader: uses ATP hydrolysis to unwind the parent strand and generates single stranded templates bound by RPA SSBs.

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Primase

Eukaryotic clamp loader: pol alpha synthesizes short primers and continues to unwind parent strands while the PCNA-RFC-Pol delta complex extends the short primers.

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PCNA-RFC-Pol delta complex

Eukaryotic clamp loader: extends short primers to generate leading strands at each replication fork + generates lagging strand okazaki fragments (ligated)