Species Diversity: intro

SBI3U (Secours)

Label the microscope

1. Body tube

2. Revolving nose piece

3. Low objective lens

4. Medium objective lens

5. High objective lens

6. Slide clips

7. Diaphragm

8. Light source

9. Occular lens

10. Arm

11. Stage

12. Coarse adjustment knob

13. Fine adjustment knob

14. Base

How to properly carry a microscope

Holding the arm and supporting the base

Lenses should always be cleaned with ______. Why?

Lens paper, it avoids leaving particles behind

When placing the coverslip on the slide, avoid the formation of __________

Air bubbles

The 3 magnifications linked to the 3 objectives are:

4x, 10x, 40x

Always start by using ____ power magnification

Low

Be sure to _____ and ____ the specimen before changing objective to a higher power

Center, focus

When using high-power lens, ALWAYS focus with the ___________

Fine-adjustment knob

To avoid damage, store the microscope with ______ power objective

Low

rules for creating biological drawings

• Unlined white paper

• Pencil

• Use compass/petri dish and ruler

• Use stippling method

• Labels on the right

When the blade is moved to the right, it appears to move towards the _____

left

Depth of Field

the depth of the object that is in focus at any one time

Field of View

visible portion of a specimin viewed through the eyepiece of an optical microscope

The eyepiece has a ______ magnification of _____

constant, 10x

Total Magnification

ocular magnification × the magnification of the lens in use

As the magnification increases, the field of view _________

decreases

mm to μm

×1000

Field of view diameter at low, medium, and high power

4mm, 2mm, 0.4mm

Fit Number

estimate of the number of times (length) the specimen can be placed within the field of view

Actual Size (formula)

A.S. = field of view diameter ÷ fit number

Scaled Ratio (formula)

S.R. = length of the cell/structure drawing ÷ A.S.

Where is the scaled ratio shown?

next to the title of the drawing