3A.2 - Enterobacteriaceae Biochemical Tests

triple sugar iron

TSI is short for:

sucrose, lactose, glucose

The TSI media contains these three carbohydrates:

0.1

TSI media contains ___% glucose.

1

TSI media contains ___% lactose.

1

TSI media contains ___% sucrose.

peptones

TSI media contains 2% of this ingredient, which provides energy for organisms that cannot use the carbohydrates:

phenol red

The pH indicator in the TSI media is:

yellow

The TSI media turns this color when it is acidic:

red

The TSI media turns this color when it is alkaline:

sodium thiosulfate

Some organisms are able to break this TSI ingredient down into hydrogen sulfide:

iron salts

This TSI ingredient reacts with hydrogen sulfide to produce a black precipitate:

beta galactosidase

This enzyme is required to catabolize lactose:

sucrase

This enzyme is required to catabolize sucrose:

slant

The arrowed section of the media is known as the:

butt

The arrowed section of the media is known as the:

A/A

Report this TSI tube:

K/K

Report this TSI tube:

K/A

Report this TSI tube:

A/H2S

Report this TSI tube:

H2S/H2S

Report this TSI tube:

acidic

It is safe to assume that media that is black is also:

inoculating needle

TSI media is inoculated using a(n):

loose fitting

The cap of a TSI tube must be ______ in order for the media to work.

18-24

TSI media should be read after ____-____ hours of incubation.

ferment glucose

Because all enterics are able to _____, they are expected to at least have a yellow butt.

non-fermentative bacillus

GNRs that cannot ferment any of the carbohydrates in TSI, and thus are K/K, are classified as:

Pseudomonas

A common genus of non-fermentative bacillus is:

proteus, salmonella, edwardsiella

Organisms in these genera can all produce hydrogen sulfide:

citrobacter freundii

This species is the only one in its genus to be able to produce hydrogen sulfide:

K/A

Proteus vulgaris, Serratia marcescens, and Yersinia entericolitica are only able to utilize glucose- which is quickly used up in the slant leading to this TSI pattern:

kliger iron agar

KIA is short for:

lysine iron agar

LIA is short for:

indole

Identify this test:

tryptophan deaminase

The indole test looks for the presence of this enzyme:

indole, kovac's reagent

The combination of these chemicals causes the color change in the indole test:

MIO, SIM

These media contain tryptophan for the indole test:

indole positive

Report this indole test:

indole negative

Report this indole test:

overnight, air

The indole test should be incubated _____, in a(n) _____ incubator.

citrate

Identify this test:

sole carbohydrate source

The point of the citrate test is that citrate is the:

pH

The color change in the citrate test is caused by a change in:

citrate positive

Regardless of color, any growth on citrate media should be considered:

citrate positive

Report this citrate test:

citrate negative

Report this citrate test

decarboxylase test

Identify this test:

ornithine, arginine, lysine

The decarboxylase test can be performed for any of these amino acids:

bromcresol purple

The pH indicator in an MIO tube for ornithine decarboxylase is:

putrescene

Decarboxylation of ornithine produces this compound:

decarboxylase positive

Report this decarboxylase test:

decarboxylase negative

Report this decarboxylase test:

mineral oil

Some _____ should be added on top of a decarboxylase tube.

glucose

MIO tubes contain a small amount of ______ to jumpstart growth.

yellow

Organisms which can only metabolize glucose will produce a(n) ______ MIO tube.

urease

Identify this test:

ammonia

The enzyme urease converts urea to ______, H₂O, and CO₂.

phenol red

The pH indicator of the urease test is:

urease negative

Report this urease test:

urease positive

Report this urease test:

phenylalanine deaminase

Identify this test:

phenylpyruvic acid

Phenylalanine deaminase converts phenylalanine to:

ferric chloride

After overnight incubation, this reagent is added to PAD tubes:

pad negative

Report this phenylalanine deaminase test:

pad positive

Report this phenylalanine deaminase test:

dnase

Identify this test:

nucleotides

The conversion of DNA to ______ causes a change in pH and thus a pinkish hue on DNase test plates.

toluidine blue

The pH indicator of the DNase test is:

48

The DNase test may take up to ______ hours to develop.

dnase negative

Report this DNase test:

dnase positive

Report this DNase test:

motility

Identify this test:

flagella

The motility test determines whether or not an organism has:

clouding

Organisms that are motile will grow all through the motility media, resulting in a(n) _______ of the agar.

motility positive

Report this motility test:

motility negative

Report this motility test: