micro review exam 3

What does Simmons' citrate test assess in bacteria?

It assesses whether bacteria can use citrate as a carbon source.

What color indicates a positive reaction in the Simmons' citrate test?

Blue indicates a positive reaction.

What are the four steps of a Gram stain procedure?

1. Crystal violet 2. Iodine 3. Ethanol 4. Safranin

What does a positive catalase test indicate?

It indicates the presence of the catalase enzyme, which produces bubbles when hydrogen peroxide is present.

What does a Spirit blue plate test for?

It tests if bacteria can produce lipase.

What would indicate a positive result on a Spirit blue plate?

A dark blue color around the bacteria indicates a positive result.

What does a capsule stain check for?

It checks to see if bacteria have capsules.

What does a black color in a SIM test indicate?

It indicates that the bacteria used cystine to produce hydrogen sulfide.

How is a positive indole test determined?

A positive indole test is determined by a red coloration at the top after adding Kovac's reagent.

What does the Voges-Proskauer test check for?

It checks if the bacteria produce acetoin from fermentation.

What color indicates a positive methyl red test?

Red indicates a positive methyl red test.

What does the casein/milk plate test for?

It checks if bacteria produce caseinase.

What does the starch plate indicate if a halo is present?

It indicates that the bacteria produce amylase and can break down starch.

What does the phenylalanine slant test assess?

It assesses whether bacteria produce phenylalanine deaminase.

In an OF glucose test, what does yellow indicate?

Yellow indicates that the bacteria can ferment or oxidize glucose.

What does the urea test evaluate?

It assesses whether bacteria produce urease, which turns the medium basic.

How is a positive nitrate test result denoted color-wise?

A red color indicates a positive nitrate test.

What does a decarboxylation test assess in bacteria?

It assesses whether bacteria can produce decarboxylases from amino acids.