Culturing microorganisms

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6 Terms

1
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How to culture microorganisms safely

Use sterilised Petri dishes and agar, inoculate using a sterile loop, seal lid with tape, incubate at 25°C.

2
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Why is culture incubated at 25°C in school labs?

Reduces risk of growing harmful human pathogens.

3
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Why sterilise equipment before use?

To kill any contaminating microbes.

4
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Why tape but not seal Petri dish completely?

Allows oxygen in to prevent growth of anaerobic, potentially harmful bacteria.

5
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How to calculate area of clear zones (antibiotic effect)

Area = π × radius².

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