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Bacteria
Prokaryotes with peptidoglycan cell walls; diverse metabolic pathways; no nucleus.
Archaea
Prokaryotes without peptidoglycan; unique membrane lipids; many extremophiles; closer to Eukarya than Bacteria.
Organisms with nuclei and membrane-bound organelles; includes plants, fungi, animals, protists.
Not included in the tree of life because they are acellular, lack metabolism, and cannot reproduce independently (require host cells).