rflp quiz

what’s a dna fingerprint?

a pattern of bands made up of specific fragments from an individuals DNA

method for producing a dna fingerprint?

restriction fragment length polymorphism (RFLP) analysis

gel electrophoresis?

All DNA fragments are loaded at the top of the gel. once electricity is run through the get the smaller DNA fragments will travel further than larger DNA fragments

Step 1:

the DNA sample is first cut into many fragments by a restriction enzyme

step 2:

the DNA fragments are then separated according to their size by gel electrophoresis (smaller goes farther)

Step 3:

radioactive probes are added and bind to DNA fragments to help them become visible

Step 4:

photographic film is applied to take a picture of DNA fragments

Step 5:

photographic film is developed

what is this technology used for?

convicting criminals from bloodstains and determining the origin of diseases