Speech Lock In

Found Research Papers

To start off, we used websites like PubMed and Google Scholar to conduct research on mitochondrial processes.

Chose Strains

From there, we selected strains of interest based on their enhanced mitochondrial quality control

Chose Genes

We selected genes of interest involved in the mitochondrial processes.

Used Principal Component Analysis (PCA)

 

We used a PCA plot to reduce the amount of variables that make up the variation, so the strains would be easier to compare.

Constructed Phylogenetic Tree

We constructed a phylogenetic tree for the C. elegans species, to understand the genetic divergence among wild C. elegans strains.

Navigated UCSC Genome Browser

 

We used the UCSC Genome Browser to locate our genes of interest and the SNPS they contain.

Located SNPs

 

SNPS are changes in the DNA sequence of a single nucleotide, and they are important to catalog because they can result in a protein change or change in gene regulation. 

Used VEP

 

We used VEP to see how these SNPs might affect protein function.

Run dN/dS analysis

We then calculated something called the dN/dS ratio for each of our genes, to see if nature was pressuring our genes to evolve. 

Used RNA Sequencing Data

 

We analyzed RNA-seq data by coding computational algorithms in order to calculate gene expression levels in different strains.