WEEK 1 - MODX LAB (SAFETY PRECAUTIONS)

Anteroom

Special area in molecular biology

Donning

Act of placing on an item, such as gloves; dressing.

Doffing

taking off

DOH

What agency regulated the hand hygiene before donning?

Gown and gloves

In CDC, the __ and ___ must be removed first

1. Agent
2. Reservoir
3. Portal of exit
4. Mode of transmission
5. Portal of entry
6. Susceptible host

6 chain of infection

40-60 seconds

How long should you wash your hands for according to CDC?

15-20 seconds

How long should you wash your hands for according to DOH?

1. Dry heat
2. Moist heat

2 heat technique

Steam underpressure for 15mins, 15psi, 121C

Principle of autoclave

1. 15mins
2. 15psi
3. 121C

3 parameters of Autoclave

Spores

Part of bacteria that is used for survival

Filtration

A process that separates materials based on the size of their particles, because liquids and solvents cannot be autoclave, therefore we should use:

Sanitize

Reduce the number of microorganisms to safe hygienic level, less effective than disinfection

Hydrogen peroxide gas

Space decontamination

1. Ionizing
2. Nonionizing

2 types of radiation

Ionizing radiation

High amount of energy, high amount of frequency and shorter wavelengths is what type of radiation?

Nonionizing radiation

Lower energy, lower frequency, and longer wavelength is what type of radiation?

1. Gamma rays
2. x-rays

2 examples of ionizing radiation

1. UV light

1 example of nonionizing radiation

1. Elimination of thymine
2. Formation of thymine dimers

2 mode of action of radiation:

1:100 (0.1%)

Dilution of sodium hydrochloride for general disinfection:

1:10 (1%)

Dilution of sodium hydrochloride for thorough disinfection

Low level disinfection

Type of disinfection that kills some fungi and viruses, and most bacteria, but is not effective against spores and TB

Intermediate level disinfection

Type of disinfection that kills most microorganisms including bacteria, most viruses and fungi, and TB and HBV; not effective against spores

High level disinfection

Type of disinfection that kills all forms of organisms for hospital and laboratory purposes

Biosafety

Used to protect people from organisms

Biosecurity

Safe keeping of valuable biological agents againts people

1. Facility design
2. Safety equipment
3. Standard and special microbiological practices

3 strategies of biocontainment

1. Primary biocontainment
2. Secondary biocontainment

2 types of biocontainment

Risk group 1

-A microorganism that is unlikely to cause human or animal disease
-EG: bacillus, adeno virus, K12 strain

Risk group 2

-Moderate individual risk, low community risk
-S. Coli
-O15H7 strain
-S. Aureus
-Adenovirus
-Salmonella

Risk group 3

-High individual risk, low community risk
-M. Tuberculosis
-B. Anthrasis
-HIV

Risk group 4

-High individual and community risk
-Ebola virus
-Margburg virus
-Lassa virus

1. Practice and procedures
2. Safety equipment
3. Facility design and construction
4. Increasing levels of protection

4 principles of biosafety

Biorisk

Biosafety + Biosecurity = ______

Proide the highest practical protection and the lowest practical exposure

Goal of Biorisk Management

1. Assessment
2. Mitigation
3. Performance

AMP model: