chapter 15 - bone marrow examination

flat bones

location of most hematopoietic tissue found in most adults

posterior iliac crest

preferred bone marrow collection site

morphology of abnormal cells

aspirate should be examined under low power to assess EXCEPT

1.5:1 - 3.3:1

normal myeloid-to-erythroid (M:E) ratio range in adults

polychromatophilic & orthochromic normoblasts

most common erythroid stages found in bone marrow of adults

osteoblasts

cells that are occasionally seen in bone marrow biopsy specimen

responsible for formation of bone

megakaryocyte

largest hematopoietic cell found in normal bone marrow aspirate

anemia with RBC indices corresponding to low serum iron & low ferritin levels

NOT an indication for bone marrow examination

1.5:1

in bone marrow biopsy specimen,

RBC precursors were estimated to account for 40% of cells

60% — granulocyte precursors

what is the M:E ratio

osteoclasts

on a bone marrow core biopsy sample — several large cells with multiple nuclei were noted; located close to the endosteum; nuclei were evenly spaced throughout the cell

what are these cells

permits assessment of the architecture & cellular rearrangement

advantage of core biopsy bone marrow specimen over an aspirate is that the core biopsy specimen:

paratrabecular lymphoid aggregation

feature that suggest involvement of the marrow by lymphoma

plasma cell

using Wright-Giemsa stain, the morphologic description of an

eccentric dark nuclei

deeply basophilic (blue) cytoplasm

prominent pale central Golgi apparatus best describes a(n)