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Roles of DNA (Key concept)
DNA molecule must be capable of storing and copying genetic information as well as putting that info to work in gene expression
Three components of nucleotide
Each nucleotide is made up of three components, five carbon sugar called deoxyribose, phosphate group, and a nitrogenous space nucleotides join together to form strands of DNA
Three components of DNA
DNA is made of nucleotides joined into long strands by covalent bonds.
Four nitrogen bases
How to adenine guanine cytosine and thymine refers to them as base names A G C and t. Any Base sequence is possible
Chargaffe’s rule
In any organism, the amount of Guanine should be equal to cytosine and the amount of Adanine should be equal to thymine
Franklins. Discovery of the structure of DNA.
1950s British scientist, Rosalind Franklin begin to study DNA and used x-ray diffraction to find DNA having a helix structure
Who were Watson and crick ? (key concept?)
Data in franklins x-ray pattern enabled Watson and crick to build first model that explains specific structure and properties of DNA
Double helix model (key concept)
Double helix model explains chargaff’s rule of base pairing, and how the two strands of DNA are held together
Antiparallel strands
Two strands of DNA run in opposite direction each strand carries a sequence of four letters
Hydrogen bonds
Hydrogen bonds hold Force just enough to hold two strands together weak forces, but only form with A-T and G-C
Why is a hydrogen bond needed to hold the most important molecule together?
It is important to have hydrogen bonds hold each of the molecules together because they’re stable and flexible
Base pairing
Hydrogen bonds form only with only certain base pairs AT and GC nucleotides are base pairing
Replication
Before a cell divides it copies it’s DNA into a copying process
DNA polymerase
The principal enzyme involved in DNA replication
Prokaryotic DNA replication
Replication in the most prokaryotic cells start from a single point, and proceeds in 2 directions until chromosome is copied fully
Eukaryotic DNA replication
Replication begins at dozens or hundreds of places on DNA molecules. Proceeds in both direction until each chromosome is copied.
RNA
Ribonucleic acid helped to put genetic code into action
DNA to RNA key difference
Unlike DNA, RNA likes to use sugar ribose instead of deoxyribose, RNA is single stranded and contains uracil in place of thymine
MRNA
Molecules of RNA, that carry copies of instructions to ribosomes from the nucleus in cytoplasm
rRNA
The subunits are made of several rRNA molecules, and as many as 80 proteins
tRNA
During assembly of proteins, a third type of RNA molecule carries amino acids to ribosomes and matches them to the coded MRNA message
Transcriptions
The process of copying a base sequence from DNA to RNA
RNA polymerase
Transcription is carried out by an enzyme, called RNA polymerase
Promoters
Regions of DNA with specific base sequences that can find to RNA polymerase
Exons
Remaining pieces are then spliced back together to form a final mRNA
Polypeptides
Proteins are made by joining amino acids together into Chains
Genetic code
Four bases of RNA form a kind of language with just four letters A C G T
Codon
Consist of three consecutive bases, that specify a single amino acid to be added to polypeptide chain
Translation
The decoding of an mRNA message into a protein
Anti-codon
Each tRNA molecules has three unpaired bases that are together
Operon
A group of genes that are regulated together
Differentiation
Gives rise to all of the specialized tissue and organs
Environmental influences affect on gene expression
Environmental changes affect genes incredibly environments can change a gene’s availability and can influence the epigenetic marks that are attached to chromatin