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27 Terms
Sheep Blood Agar (CBA) - Classification
Nutritive, differential, enriched (?)
Sheep Blood Agar Ingredients
Defibrinated sheep blood, agar
Why is SBA considered enriched?
It has sheep’s blood
Why is SBA considered nutritive?
The blood provides nutrients for organisms
Why is SBA considered differential?
Blood allows for visualization of hemolysis
Chocolate Blood Agar (CBA) - Classification
Enriched, nutritive
Why is CBA considered enriched?
It has sheep’s blood added and lysed, providing hemoglobin products and NAD
Why is CBA considered nutritive?
The sheep’s blood supports most organisms
MacConkey Agar (MaC) - Classification
Differential, Selective
Why is MaC considered differential?
Lactose is added to differentiate lactose fermenters from non-lactose fermenters
Why is MaC considered selective?
Bile salts and crystal violet are added to inhibit gram positives
Eosin Methylene Blue Agar (EMB) - Classification
Selective, differential
Why is EMB considered selective?
Eosin Y and methylene blue are added to inhibit gram positives
Why is EMB considered differential?
Lactose and sucrose are added to distinguish fermenters
Colistin Nalidixic Acid Agar (CNA) - Classification
Enriched, selective
Why is CNA considered enriched?
It has sheep blood added
Why is CNA considered selective?
Colistin and nalidixic acid are added to inhibit gram negatives
Thioglycollate Broth - Classification
Nutritive, enriched
Why is thioglycollate broth considered nutritive?
Peptone, sugars, yeast extract, and NaCl are added to support organism growth
Why is thioglycollate broth considered enriched?
Can add supplements (e.g. more reducing agents) to support different organisms
What organisms grow on SBA?
Most medically significant bacteria, including fastidious bacteria
What organisms grow on CBA?
Facultative Anaerobes
Fastidious organisms
Neisseria (with CO2)
What organisms grow on MaC?
Gram negative bacteria, particularly enterics (Enterobacteriaceae)
What organisms grow on EMB?
Gram negative bacteria, especially enterics (Enterobacteriaceae)
How does EMB differ from MaC?
Lactose fermenters grow DARK on EMB, and pink/red on MaC
What organisms grow on CNA?
Gram positive cocci, especially Enterococcus
What organisms grow well in thioglycollate broth?
Most organisms
O2 spectrum makes it good for detecting oxygen needs