6 - HEMOLYSIS, NEUTRALIZATION, AND COMPLEMENT FIXATION TEST

Hemolysis

Alteration, dissolution, or destruction of red blood cells in such a manner that hemoglobin is liberated

Neutralization

Process of inactivation by the presence of antibodies that are known as neutralizing antibodies, antibodies that can inhibit the infectivity of a virus or the toxicity of a toxin molecule

Complement Fixation

The triggering of the classical complement pathway due to combination of antigen with specific antibody

15

fresh

Procedure

1. Label the tubes as 1A to 6A and 1B to 6B

2. Proceed as follows:

HEMOLYSIS TEST
Tube Number	1A	2A	3A	4A	5A	6A
Fresh serum (drops)	0	1	2	3	4	5
RBC suspension (drops)	2	2	2	2	2	2

3. Mix well; centrifuge for _ seconds in serofuge

4. Do not disperse the sediments. Observe for a change in the supernatant

5. Follow the same procedure for tubes 1B to 6B, this time substitute the aged serum for _ serum. Using a vortex

6. Fresh serum with aged serum

Clear, colorless supernatant

Interpretation of Result (Hemolysis)

Negative

Pink to faint red supernatant

Interpretation of Result (Hemolysis)

Positive

Complement Fixation Test

Test to detect complement-fixing antibody

Bacterial Antigen

Complement Proteins

Indicator Red Cells (Sensitized RBC)

Complement Fixation Test

Procedure

Serum plus Reagent 1: _ _

Plus Reagent 2: _ _

Plus Reagent 3: _ _ _

Pink to faint red supernatant

Interpretation of Result (Complement Fixation Test)

Negative

Clear, colorless supernatant

Interpretation of Result (Complement Fixation Test)

Positive