subcelluar fractionation

subcellluar fractionation

uses differential centriguation to seprate organelles via density

what organells are we isoalting?

mitchondria and nuecleus

what are componets of subcelluar fractionation?

size and desnity

at low speeds….

the desnest fluids will be at the bottom and from a pellet

the less desnse fluids would need to be spuna t higher cenitrgal speeds

….

what contained our nueclei

pellet 1

what contianed out mitchondria ?

pellet 2

whats the need for azure dye?

used to stain chromomes in the NUCELI

SDH catalyzes the reduction of succinate to fumerate

true

sdh is covalent bound to one molecule of FAD

yes

what does azide do?

blocks the flow of electrons

whats dcip

artiical electorn acceptors that takes the palce of FADH2