Amino acid sequencing steps

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From slide 81

Last updated 5:25 PM on 7/13/26
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8 Terms

1
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1.

Separate polypeptide chains

2
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2.

Cleave (reduce) disulfide bridges

3
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3.

Determine composition of each chain

4
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4.

Determine n and c terminal residues

5
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5.

Cleave each chain into smaller sequences and determine sequence of each

6
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6.

Repeat 5 with a different cleavage procedure

7
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7.

Reconstruct the sequence of the protein from sequences of overlapping fragments

8
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8.

Determine positions of disulfide crosslinks