Bioreactor Design and RNA Extraction Techniques

Bioreactor

Device supporting a biologically active environment.

Aerobic process

Chemical process requiring oxygen for organisms.

Anaerobic process

Chemical process occurring without oxygen.

Static bioreactor

Culture method lacking mechanical stimulus.

Dynamic bioreactor

Culture method with active mechanical stimulus.

2D culture

Flat culture method, less physiological relevance.

3D culture

Culture method mimicking in vivo conditions.

Cell type

Specific cells used in bioreactor experiments.

Culture condition

Environmental factors affecting cell growth.

Matrix composition

Materials forming the structure for cell growth.

Sterility

Absence of living microorganisms in bioreactor.

Cytotoxicity

Toxic effects of substances on living cells.

Shear stress

Force per unit area acting on cell surfaces.

Young's modulus

Measure of stiffness of a material.

IC50

Concentration inhibiting 50% of cell growth.

RNA extraction

Process isolating RNA from biological samples.

qPCR

Quantitative polymerase chain reaction for gene analysis.

RNA sequencing

Technique for analyzing the complete RNA profile.

Transcriptomics

Study of RNA transcripts in a cell.

Phase separation

Process separating RNA from proteins and DNA.

Spectrophotometry

Method measuring RNA concentration and purity.

Fluorometric quantification

Sensitive method for measuring RNA concentration.

RNA Integrity Number (RIN)

Quality metric for RNA samples, ≥7 preferred.

Caspase 3

Enzyme indicating cell apoptosis or death.

Ki67

Marker indicating cell proliferation.

PLAU expression

Gene expression related to enzyme activity.

Mechanical disruption

Method to break cells for RNA extraction.

Ethanol precipitation

Technique to concentrate RNA during extraction.

Contamination assessment

Evaluation of RNA purity using A260/A280 ratio.