enzymes - assay enzyme activity

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assay enzyme activity of each enzyme (methodologies)

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11 Terms

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CK

catalyzes both forward and reverse reactions involving phosphorylation of creatinine of ADP

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LD

catalyzes interconversion of lactic and pyruvic acids using a coenzyme NAD, in either forward or reverse direction

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AST

based on Karmen Method

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ALT

coupled enzymatic reaction using lactate dehydrogenase as indicator enzyme, which catalyzes reduction of pyruvate to lactate with simultaneous oxidation of NADH

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ALP

catalyzes the reaction of p-Nitrophenyl phosphate to p-Nitrophenol plus phosphate at a pH of 10.2

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ACP

activity found primarily in the prostate

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amylase

activated by Ca and Cl and catalyzes the breakdown of starch and glycogen into glucose, maltose, and dextrins

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lipase

hydrolyzes the ester linkages of fats to produce alcohols and fatty acids

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GGT

detoxification processes in the liver

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G6PD

helps protect red cells from oxidative damage

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PSA

found the prostate, helps man stuff