Anatomy of Prokaryotic Cell pt. 2

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Last updated 4:10 AM on 9/4/26
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6 Terms

1
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Three types of Staining

  1. Simple

  2. Differential

  3. Special


2
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Simple Stains

Aqueous or alcohol solution of a single basic dye

Purpose: Highlight the entire microorganism so that cellular shapes and basic structures are visible

Procedure: Stain is applied to the fixed smear → washed off → dried & examined

3
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Mordant

Chemical added to a simple stain solution to intensify the stain

Coat structures (flagellum) to make it thicker & easier to see after it’s stained with a dye

Ex: CV, carbolfuchsin, methylene blue, safranin

4
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Gram Staining (Differential) - WIP

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2)

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5
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Differential Stains

React differently with each type of bacteria and thus can be used to distinguish them

Ex) Gram-stain & Acid-fast stain

6
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Acid-Fast Stain

Bind ONLY to bacteria with waxy material