Visualising cells - fluorescence microscopy, fluorescent probes and proteins

use of fluoresences

• transmission light microscropy only reveals certain organelles

• (EM) revealing the complex arrangement of organelles within cells

• cannot easily use EM to studying indiviual organelles and can not use to studying living cells

how fluroescense work

fluorscent molecules work by absorbing light at one wavelength and emitting light at another wavelength

immonflurensencts

attaching a fluroscences marker to an antibody that recongise a certain protein → that can be studied

GPF tagging

using molecular biology to fused DNA that has the protein to GFP. this genetic fusion is used to to put back into a cell to be transcribed and translated. this protein is now expressed

what are the advantages of GFP

they can be visusallsized in living system