BLOOD BANK

should be more than 1 year

Donor Deferral

Tattoo/Piercings

2 hrs after heavy meal

Donor Deferral

Last meal

24 hrs from the last intake

Donor Deferral

Alcohol intake

7 days before and after

Donor Deferral

Menstruation

At least 6 hours

Donor Deferral

Hours of sleep

2 hrs after

Donor Deferral

Smoke/vape

50 kg or 110 lbs

Donor Vitals

Weight

37.5 deg C / no fever

Donor Vitals

Temperature

50-100 in 1 minute *repeat if high or low*

Donor Vitals

Pulse

100-140 mmHg

Donor Vitals

Blood Pressure

Systolic

70-90 mmHg

Donor Vitals

Blood Pressure

Diastolic

12.5-17.5 g/dL

Donor Vitals

Hemoglobin

Female

13.5-17.5 g/dL

Donor Vitals

Hemoglobin

Male

Alcohol – Iodine – Alcohol

Blood Donation

Aseptic Technique

3 Micropores

1 Stepty

1 Lavender top (5mL)

1 Yellow Top (5 mL)

1 Red Top (10 mL)

Blood Donation

Materials for bleeding (5)

3500-4000 rpm, 10 minutes, 22 C

Blood Processing

Centrifuge the Whole Blood

Program 1

1000 rpm, 5 minutes, 22 C

Blood Processing

Centrifuge the Whole Blood

Program 2

3900-4300 rpm, 10 minutes, 22 C

Blood Processing

Centrifuge the Whole Blood

Program 3: Hard Spin

1000 rpm, 5 minutes, 22 C

Blood Processing

Centrifuge the Whole Blood

Program 4: Soft Spin

2500 rpm, 10 minutes, 6 C

Blood Processing

Centrifuge the Whole Blood

Program 5: Cryoppt/washing of PRBC

Compomat G5

Optipress

Blood Processing

Place the primary bag on the _ _ or _ to start processing the blood. Please see the workflow attached and follow the steps for whole blood processing

30

Blood Processing

Blood bags are weighed by subtracting _ cc

30, 1

Blood Processing

Platelets are hanged for _ minutes to_ hour after Program 2

3 disposable tubes, 1 disposable pipette, buffered saline, and antisera; A, B, and D

Forward Typing

Materials (4)

1

1-2

1

Forward Typing

1. Label the tubes with donors serial or name and A, B, and D

2. Put _ mL buffered saline in the tube labeled as A

3. Get _-_ drops of whole blood or packed red cell from the lavender top and place it in tube A

4. Mix and place _ drop of red cell suspension into tube A, B, and D

1

30, 3,400

Forward Typing

1. Place _ drop of anti-sera, Anti-A, Anti-B, and Anti-D into the corresponding labeled test tube

2. Mix the content gently and centrifuge for _ seconds at approximately _ rpm

3. Gently agitate each tube to resuspend the cell buttons and examine for agglutination

4. Read and interpret the result 

5. Let the staff also check the resultsresults

Positive

Negative

FORWARD TYPING
Blood Group	Anti-A	Anti-B
A	_	_

Negative

Positive

FORWARD TYPING
Blood Group	Anti-A	Anti-B
B	_	_

Negative

Negative

FORWARD TYPING
Blood Group	Anti-A	Anti-B
O	_	_

Positive

Positive

FORWARD TYPING
Blood Group	Anti-A	Anti-B
AB	_	_

Positive

Negative

Anti-D with Agglutination	_
Anti-D without Agglutination	_

2 tubes, 1 pipette, and known A and known B

Reverse Typing

Materials (3)

2

1

30, 3,400

Reverse Typing

1. Label the test tube with the donor or patient’s name, A and B

2. Place _ drops of plasma or serum into the tubes

3. Place _ drop of known A and known B

4. Mix the content gently and centrifuge for _ seconds at approximately _ rpm

5. Gently agitate each tube to resuspend the cell buttons and examine for agglutination

6. Read and interpret the result

7. Let the staff also check the results

Negative

Positive

REVERSE TYPING
Blood Group	Known A	Known B
A	_	_

Positive

Negative

REVERSE TYPING
Blood Group	Known A	Known B
B	_	_

Positive

Positive

REVERSE TYPING
Blood Group	Known A	Known B
O	_	_

Negative

Negative

REVERSE TYPING
Blood Group	Known A	Known B
AB	_	_

Parascreen individual pouch (5 uL loop and test device) and clearing buffer

Malarial Screening Test

Materials (2)

5

2

20

Malarial Screening Test

1. Mix anticoagulated tubes

2. Collect _ uL of whole blood to be tested using a sample loop

3. Blot the blood in the sample port A

4. Hold the clearing buffer vertically over port B and add _ drops

5. Start the stopwatch

6. Read the results after _ minutes

COM.TEC (Fresenius Kabi)

Continuous Centrifugation

N/A

MACHINES/ANALYZERS
	Principle	Sample	Use
_	_	_	For platelet pheresis procedure, therapeutic plasma exchange, and stem cell collection

ARCHITECT Plus

Chemiluminescence

Yellow top (5 cc)

MACHINES/ANALYZERS
	Principle	Sample	Use
_	_	_	Serological screening (HIV, HBsAg, HCV, Syphilis)

Procleix Panther System (NAT Testing)

Transcription Mediated Amplification

Red top (10 cc)

MACHINES/ANALYZERS
	Principle	Sample	Use
_	_	_	Serological screening

Ortho-vision

Column Agglutination

Lavender top (5 cc)

MACHINES/ANALYZERS
	Principle	Sample	Use
_	_	_	Crossmatching, blood typing, antibody screening, and identification

Compomat G5

N/A

Whole blood (450 mL)

MACHINES/ANALYZERS
	Principle	Sample	Use
_	_	_	Processing of blood (PRBC, FFP, and PLT conc)

Cryofuge16 and ROTO SILENTA

N/A

Whole blood (450 mL)

MACHINES/ANALYZERS
	Principle	Sample	Use
_	_	_	Centrifugation of whole blood, processing of cryoppt, and washing of red blood cells

80

1-6

21

35

42

1-6

24

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Red Blood Cells (Packed Cells)	Hct _% (maximum)	Closed System: _ C CPD: _ days CPDA-1: _ days Additive: _ days Open System: _ C _ hours

85

<5x10^6

1-6

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Leukocyte Reduced Cells	_% of original cells _ Leukocytes in 95% units tested	_ C, same as red cells

>80

glycerol

-65, 40

-120, 20

24

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Frozen Red Cells	_% of original red cells, adequate removal of cryoprotective agent (_)	10 years: _ C or colder (_% glycerol) _ C (_% glycerol) _ hours once deglycerolized

≤-18, ≤-65, 6-8

12, <-18

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Fresh Frozen Plasma	Frozen to _ C or _ C within _-_ hours (depends on anticoagulant)	_ months, _ C

≥80

150

12, ≤-18

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Cryoprecipitated AHF	_ IU/unit x number of components in pool _ mg fibrinogen	_ months, _ C

≥5.5x10^10, 90

6.2, 90

3/5

20-24

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Platelets, Single Donor (Closed System)	_ plt/unit in _% of units tested pH _ or greater in _% units tested at maximum storage time	_/_ days (depends on collection bag) _-_ C with constant agitation

closed system

4, 20-24

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Platelets, Pooled (Open System)	Same as _ _	_ hours, _-_ C with agitation

≥5.5x10^10, 75

≤8.3x10^5, 95

<5x10^6

platelets

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Platelets, Leukocyte-Reduced	_ platelets in _% of units tested _ leukocytes in _% of units tested _ in pooled platelets	Same as _

≥3.0x10^10, 90

6.2

5, 20-24

24

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Platelets, Pheresis	_ plt/unit in _% of units tested  pH _ or greater at maximum storage time	_ days, _-_ with constant agitation _ hours (open system)

≥1.0x10^10, 75

24, 20-24

EXPIRATION OF BLOOD PRODUCTS AND BLOOD COMPONENT STORAGE
Component	Standards	Storage
Granulocytes, Pheresis	_ granulocytes in _% of units tested	_ hours, _-_ C

8 hours

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Thawed Cryoprecipitate	_

1 year

-18 C to -60 C

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Fresh Frozen Plasma (Frozen)	_	_

21 days

1-6 C

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Packed RBC with CPD	_	_

42 days

1-6 C

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Packed RBC with SAGM filter	_	_

35 days

1-6 C

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Packed RBC with CPDA1	_	_

28 days

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Irradiated Packed RBC	_

5 days

20-24 C with constant agitation

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Platelet Concentrate	_	_

24 hours

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Washed Red Blood Cells	_

5 days

1 C to 6 C

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Thawed Fresh Frozen Plasma	_	_

-40 C (Biofreezer)

BLOOD COMPONENTS, SHELF LIFE, AND STORAGE TEMPERATURE
Blood Component	Shelf Life	Storage Temperature
Cryoprecipitate		_

ABO

Donor Blood Processing

Resolve discrepancies

Rh

Donor Blood Processing

Weak D determination on D negatives

Antibody Screens

Donor Blood Processing

Clinically significant antibodies

FFP

platelets, cryoprecipitate

Donor Blood Processing

Antibody Screens

_ cannot be prepared from these units; _ and _ can (contain minimum plasma volume)

Syphilis

Donor Blood Processing

Serological test such as RPR

HBsAg

Anti-HBc

Anti-HCV

Anti-HIV 1/2

Anti-HTLV-I/II

Donor Blood Processing

Viral Diseases (5)

HIV-1 RNA

HCV RNA

West Nile RNA

Donor Blood Processing

NAT Testing for: (3)

Whole Blood

Blood Components

Given in cases of severe shock, rarely used due to increased use and availability of components

Packed Red Cells

Blood Components

Red cells with plasma removed

Provides the same oxygen carrying capacity as whole blood with less volume

<80

55-65

1, 3

Blood Components

_% hct indicated sufficient plasma removal

_-_% hct if additive solution is used

1 unit raises hemoglobin by _ g or hematocrit by _%

Platelets

Blood Components

Do not transfuse through a microaggregate filter

Only one ABO type/pool

5,000-10,000

Blood Components

Platelets

1 unit of platelets should raise platelet count by _-_ in average-sized adult

4

Blood Components

Platelets

Expires _ hours after pooling in an open system

Fresh Frozen Plasma

Blood Components

Used for multiple coagulation deficiencies, factor XI deficiency, and other congenital deficiencies which no concentrate is available

1, ≤-18

7, ≤-65

Blood Components

Fresh Frozen Plasma

Expires _ year from date of collection when stored at _ C or _ years stored at _ C

24

5

1-6

Blood Components

Fresh Frozen Plasma

Once thawed (between 30-37 C), it expires in _ hours (laboratory setting expiry will be _ days) and stored at _-_ C

Cryoprecipitate

Blood Components

Also contains vWF, Factor XIII, and fibronectin

≥150 mg

≥80 IU/bag

VIII

Blood Components

Cryoprecipitate

Must contain _ of fibrinogen and _ of factor _

<-18, 1

room temperature, 8

Blood Components

Cryoprecipitate

Store at _ C for _ year from date of phlebotomy; _ _ after thawing _ hours

6, 4

Blood Components

Cryoprecipitate

Transfuse within _ hours of thawing; _ hours after pooling

Irradiated Blood and Components

Blood Components

Prevents graft (donor lymphocytes) vs. host disease (GVHD) by inactivating donor lymphocytes

25, 2500

Blood Components

Irradiated Blood and Components

Minimum of _ Gy (_ cGy)

28

Blood Components

Irradiated Blood and Components

RBCs expire on original outdated or _ days after irradiation, whichever comes first