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Eukaryotic cells
replicates DNA in the nucleus
Prokaryotic cells
replicates DNA in the cytoplasm
DNA Replication
Is the copying of DNA during S-phase (DNA to DNA)
Transcription
Is the copying of genes worth of DNA on a single strand of mRNA (DNA to mRNA)
mRNA
messenger RNA- Carries the instructions for a single protein from the nucleus to the ribosome
rRNA
ribosomal RNA- Reads the mRNA instructions to assemble amino acids into proteins
RNA Polymerase
Elongates RNA in the 5’ —> 3’ direction
Stop codons/Termination signal
UAA, UAG, UGA
What is the codon table?
It’s degenerate
Mutation
Any change in the DNA sequence
What causes mutations?
Errors in replication, transcription, or cell division
What are some mutagens that can change skin, muscle or bone cells?
Radiation from the sun, X rays, and radioactive materials
Where does information flow?
it flows from DNA to mRNA to protein
Point mutation
A change in a single base pair in DNA
Frameshift mutation
involves more than a single codon, occurs when a nitrogenous base is deleted or added
Prokaryotic Replication
A circular DNA strand is opened in one origin of replication.
Eukaryotic Replication
DNA unwinds in multiple areas as its is replicated
Semi-Conservative Replication
Each new strand is composed of one original strand and one newly synthesized strand.
What are the three main stages of Semi-Conservative Replication?
Unwinding, Base Pairing, Joining
DNA Helicase
Unwinds DNA
Primase
Adds a few RNA nucleotides
DNA Polymerase
Elongates DNA to the 5’—> 3’ direction , and proofreads bases
DNA Ligase
Glues Okazaki fragments together
DNA
Deoxyribonucleic Acid
Thymine as a base pair for Adenine
2 stranded double helix
Contains all DNA
3 parts of Nucleotide
5-C Sugar
Phosphate Group
Nitrogen Base
What is DNA?
DNA is a double helix made of 2 twisted strands
What is the sugar phosphate backbone
It’s anti parallel and causes a leading and lagging strand
What is the number of hydrogen bonds that Adenine and Thymine have?
They have 2 hydrogen bonds
What is the number of hydrogen bonds that Cytosine and Thymine have?
They have 3 hydrogen bonds
What are purines?
They are double ringed
What are pyrimidines?
They are single ringed
Nitrogenous Base
A carbon ring structure that contains one or more atoms of nitrogen.
What creates the lagging strand?
The antiparallel sugar phosphate backbone
tRNA
Delivers amino acids to ribosomes to assemble proteins.
What are the three nitrogenous bases known as?
Codons
Translation
The process of changing the information in mRNA into an amino acid chain
RNA
Ribose
Uracil Substitutes for Thymine
Single Stranded
1 genes worth of DNA
3 Types of RNA
mRNA
rRNA
tRNA
Start Codon
AUG