Micro Practical 1 mini shitty

Condenser

Focuses light on specimen

Iris diaphram

Regulates amount of light and contrast

Coase focus knob

For focusing with low magnification

Fine focus knob

For focusing under high magnification

Revolving nose piece

Holds objective lenses and allows for rotation between them

Ocular lens

Lens you look through, 10x magnification

Stage

Platform where slide is placed

Objective lens magnifications

4x, 10x, 40x, 100x

Total magification equation

Objective lens x Ocular lens

What morphology?

Bacillus

What morphology?

Coccus/cocci

What morphology?

Spirillum

Cluster of four cocci is called?

Tetrad

Describe ‘strepto’ arrangment

Cells in chains

Describe staphylo arrangement

irregular group, no specific arrangement

Morphology

Cell shape and arrangement

Smear procedure

Smear → air dry → heat fix

What type of staining?

Negative staining, acidic dye (negatively charged)

What is decolorizer?

Alcohol: decolorizes gram-negative cells

Gram-negative cells…

Have a thin peptidoglycan layer and an outer membrane

What type of staining?

Capsule

What is this?

Endospore

What flagellar arrangment?

Peritrichous

Describe amphitrichous flagella

Single flagella at each end of cell

Virulence factor examples

Flagella, outmembrane,