BIOL3401 Midterm Review

which container would you place your used gloves in

biohazardous waste

which container would you put a used coverslip in

broken glass container

which container would you place a razor in

sharps container

which piece of glassware is the most precise way to measure large amounts of liquid

graduated cylinder

which piece of glassware has a progressively smaller circumference from the bottom to the top

erlenmyer flask

which piece of glassware has an equal circumference until the top where the opening has a much smaller circumference

media bottle

which piece of glassware has an equal circumference from the top to the bottom and cannot be used for precise measurements

beaker

which machine is used to grow and maintain microorganisms at optimal physical conditions

incubatorw

which piece of glassware is used to move liquid culture around an agar plate evenly

spreader

which piece of equipment is used to sterilize tools and glassware on the benchtop

microincinerator

which piece of equipment is used for visualizing microbes

microscope

which tool is used to move microorganisms between cultures and can be repeatedly sterilized

inoculating loop

which machine rotates a mixture to separate out the different parts

centrifuge

which machine is used to heat a mixture and stir a mixture using electromagnetic forces

hot plate/magnetic stirrer

which machine agitates a mixture in a test tube to make a homogenized mixture

vortex mixture

which machine is used to measure masses

analytical balance

which piece of equipment is used to avoid contamination when working with microbes

laminar flow hood

which piece of equipment is used to sterilize equipment and media

autoclave

which piece of equipment is measure the amount of material based on absorbance

spectrophotometer

which machine is used to perform polymerase chain reactions

thermocycler

which machine is used to keep liquids at a constant temperature

waterbath

which piece of glassware/plastic is to hold small amounts of liquid in molecular biology

microcentrifuge tube

which piece of glassware is used to grow microbes in liquid culture

test tube

which piece of glassware is used to grow microbes in solid or semi-solid agar

petri dish

which pipet moves a fixed volume

pasteur pipet

which pipet is used to transfer the largest amounts of liquid

serological pipet

which pipet is used to transfer the smallest amounts of liquid

micropipet

those organisms that pose very little risk of disease for healthy students, and they can still capable of causing infection under certain circumstances are rated

BSL-1

those organisms that pose a moderate risk of infection, but the diseases caused by these organisms are treatable and usually not serious are rated

BSL-2

though we will not use these organisms during lab, organisms that can cause disease in healthy adults and can spread to the community but have an effective treatment are rated as

BSL-3

though we will not use these organisms during lab, organisms that can cause disease in healthy adults, poses a lethal risk and does not respond to vaccines or antimicrobial therapies are rated as

BSL-4

growing microbes in culture is important to increase visibility and manage microbes in an artificial environment is which of the following

inoculation

the method uses media to separate out individual microbes to make pure culture is known as

isolation

this process that uses media and cultured microbes occurs when the microbes are placed under optimal growth condition to promote growth and reproduction of the microbes is known as:

incubation

this is the analysis of collected data to help determine the type of microbe that is present and is known as

identification

this type of microscope cause the light source to hit the specimen at a severely oblique angle

darkfield

this is the most common type of microscope used

brightfield

a benefit of this microscope is the ability to clearly view live organisms without stains

phase contrast

when using the scanning objective, the objective magnification would be

4x

when using the low-power objective, the objective magnification would be

10x

when using the high-dry objective, the objective magnification would be

40x

when using the oil immersion objective, the objective magnification would be

100x

which type of cell wall (Gram negative or Gram positive) was Escherichia coli

gram negative

which type of cell wall (Gram negative or Gram positive) was Pseudomonas aeruginosa

gram negative

which type of cell wall (Gram negative or Gram positive) was Bacillus subtilis

gram positive

which type of cell wall (Gram negative or Gram positive) was Stahylococcus epidermidis

gram positive

which supergroup do Kinetoplastida, Diplomonads, Parabasilids, Euglenazoans and Euglenids belong to

excavata

which supergroup do Cercozoa, Radiolarians and Foraminiferans belong to

rhizaria

which supergroup do Apicoplexans, Ciliates and Dinoflagellata belong to

alveolata

the phylum Nematoda belongs to which kingdom

animals

the phylum Platyhelminthes belongs to which kingdom

animals

which fungal phylum contains bread molds

zygomycota

which phylum contains the fungus that produces pencillin and brewer's yeast (Saccharomyces)

ascomycota

which phylum contains the plant pathogenic fungi known as smuts and rusts

basidiomycota

which virus classification incudes double stranded DNA?

class I

which virus classification includes single stranded DNA

class II

which virus classification includes double stranded RNA

class III

which virus classification includes positive sense single stranded RNA

class IV

which virus classification includes negative sense single stranded RNA

class V

which virus classification includes single stranded RNA with a DNA intermediate

class VI

which virus classification includes double stranded DNA with an RNA intermediate

class VII

which type of stain sticks to the cell and gives them color

positive staining

which type of stain does not stick to the cell, but dries around the cell boundary creating a silhouette

negative staining

which type of stain uses both basic and acidic dyes

gram staining

which type of stain uses a single dye

simple staining

which type of stain uses two dyes - a primary dye and a counterstain

differential staining

what cell structure does Gram staining react to

cell wall

what cell structure gets stained when the cell is under environmental stress

spore

what waxy material in the cell wall does acid-fast staining react to

mycolic acid

name the two stains used in Gram staining

crystal violet, safranin

name the two stains used in acid-fast staining

fuchsin, methylene blue

name the two stains used in spore staining

malachite, safranin

name the one stain that can be used in negative staining

nigrosin

which type of cell wall is indicated by purple/blue in Gram staining

gram positive

which type of cell wall is indicated by pink in Gram staining

gram negative

with spore staining, green indicates which structure

spore

with spore staining, red indicates what type of cell

vegetative cells

with acid-fast staining, Mycobacterium (acid-fast bacteria) will stain which color because of the presence of mycolic acid

red

with acid-fast staining, non-acid-fast bacteria will stain which color

blue

looking at the micrograph, is Staphylococcus aureus Gram positive or Gram negative

gram positive

looking at the micrograph, is Pseudomonas aeruginosa Gram positive or Gram negative

gram negative

looking at the micrograph, is Bacillus megaterium Gram positive or Gram negative

gram positive

looking at the micrograph, is Moraxella catarrhalis Gram positive or Gram negative

gram negative

looking at the micrograph, does the bacteria Clostridium difficile produce spores

yes

looking at the micrograph, does the bacteria Bacillus cereus produce spores

yes

looking at the micrograph, does the bacteria Staphylococcus sp. produce spores

no

looking at the micrograph, does the bacteria Bacillus megaterium produce spores

yes

looking at the micrograph, does the bacteria Mycobacterium smegmatis produce the waxy material in its cell wall

yes

looking at the micrograph, does the bacteria Bacillus cereus produce the waxy material in its cell wall

no

looking at the micrograph, does the bacteria Mycobacterium tuberculosis produce the waxy material in its cell wall

yes

these media are designed to grow a broad spectrum of microbes that do not have special growth requirements

general purpose

these media contain complex organic substances such as growth factors that are required for the microbes to grow

enriched

these media contain substances that absorb oxygen or slow the penetration of oxygen

anaerobic growth

this type of media is used to maintain and preserve the microbe for a period of time before testing

specimen transport

this type of media contains one or more agents that inhibit the growth of certain microbes but not another

selective

this type of media can grow several types of microbes but are designed to bring out visible differences among those microbes

differential

this type of media is used to count the number of microbes in agricultural, industrial or environmental samples

enumeration

this type of media is used to test the effectiveness of antimicrobial drugs, disinfectants and antiseptics

assay

this type of media contain pure chemical nutrients that vary little from one source to another and have a molecular content with an exact formula

synthetic media