Blood Donor and Selection Processing (ASCPi)

17 years old

What is the minimum donor age?

≥ 50 kg (≥110 lbs)

Weight requirement for blood donation

37.5°C (99.5 °F)

Potential donor temperature should not exceed:

• Systolic ≤ 180 mmHg

• Diastolic ≤ 100 mmHg

Blood pressure requirement for donors

≥ 12.5 g/dL

Hemoglobin requirement for blood donation

1. Hemoglobin: ≥ 11 g/dL

2. Hematocrit: ≥ 33%

3. NO BACTEREMIA

Criteria for autologous transfusion

Every 3 days but not within 72 hours of a scheduled surgery

For autologous transfusion, blood may be drawn:

Spectrophotometric

Most sensitive method of hemoglobin determination

25

30 mL of copper sulfate is equal to how many tests?

1.053

Specific gravity of Copper sulfate reagent is:

15 seconds

In Copper sulfate method, acceptable drop of blood will sink in solution within:

Preoperative

Type of autologous donation that occurs during the 5 to 6 weeks immediately preceding a scheduled, elective surgical procedure unless the red blood cells and plasma are scheduled to be frozen.

Acute Normovolemic Hemodilution

Type of autologous donation that results in the collection of whole blood with the concurrent infusion of crystalloid or colloid solutions, thus maintaining a normal blood volume but decreasing the patient’s hematocrit.

Intraoperative Collection

Type of autologous donation that involves collecting shed blood from the surgical site; processing the blood through an instrument that washes it with saline to remove tissue debris, free hemoglobin, and plasma that may contain activated coagulation factors; concentrating the residual red cells (to a hematocrit of 50% to 60%); and then reinfusing those cells immediately.

Postoperative Blood Salvage

• Type of autologous donation that involves collecting blood from a drainage tube placed at the surgical site. The collected blood is then reinfused, with or without processing, via microaggregate filter to screen out any debris.

• The blood is characterized as being dilute, partially hemolyzed, and defibrinated

Accept for whole blood donation

A donor took aspirin 24 hours ago and now wants to donate platelets. What is the appropriate action?

6 months deferral

A male donor reports taking Avodart (dutasteride) for benign prostatic hyperplasia. What deferral is required?

12 months deferral

A 45-year-old man reports having been treated for syphilis 2 years ago. He completed the full course of antibiotics. What is the appropriate deferral period?

12 months deferral

A potential donor was incarcerated in a correctional facility for 3 months and released 10 months ago. What is the appropriate action?

12 months deferral

A donor returned 6 months ago from a 2-week trip to a malaria-endemic country. She had no symptoms of malaria. What is the appropriate action

3 year deferral

A donor has taken Soriatane (acitretin) for psoriasis and stopped the medication 3 months ago. What is the correct deferral?

Permanent deferral

A donor reports that a close relative died of Creutzfeldt-Jakob Disease (CJD), but he himself is asymptomatic. What is the appropriate course of action?

Permanent deferral

A donor had been previously treated with pituitary growth hormone of HUMAN ORIGIN. What is the correct deferral?

Permanent deferral

A patient was revealed to have a history of Chaga’s disease. What is the correct deferral?

No deferral

A patient was revealed to have had their TB treated and is inactive ever since. What is the correct deferral?

18G

What is the needle gauge for blood transfusion?

16G

What is the needle gauge for blood donation?

• Platelets

• FFP

• Cryoprecipitated Antihemophilic Factor (AHF)

Blood collection that has exceeded 15 minutes may make the unit unsuitable for the preparation of:

Diversion pouch or 2nd phlebotomy

After blood collection, the sample for testing should be taken from:

24 hours

Whole blood units stored at 1-6 °C should be submitted to the blood bank/center within:

6-8 hours

Whole blood units stored at 20-24 °C should be submitted to the blood bank/center within:

Platelet preparation

Whole blood submitted within 6-8 hours after collection can be used for:

yellow

Color label for Type A blood bags

Pink

Color label for Type B blood bags

White

Color label for Type AB blood bags

Blue

Color label for Type O blood bags

1. ABO grouping

2. Rh typing

3. Antibody screen

4. Screening for transfusion-transmissible infections

Tests performed to donor blood:

6-8 hours

Separation of whole blood components should be done within:

Whole blood

What component is used for actively bleeding patients who have lost at least 25% of their blood volume, or patients that require exchange transfusion?

Packed RBCs

Which component is needed for oncology patients undergoing chemotherapy or radiation therapy, trauma patients, dialysis patients, premature infants with trauma, and premature infants with sickle cell?

Thawed AB type plasma from different donor

If whole blood is not available, you should reconstitute whole blood by mixing RBCs with:

<5 x 10^6 WBCs

1 unit of Leukoreduced RBCs contain:

Leukocyte-reduced RBCs

Which component is used for patients with severe and/or recurrent febrile transfusion reactions due to leukocyte antibodies?

Washed RBCs

Which component is used for patients with transfusion history of allergic, urticarial reaction, anaphylactic reaction, and febrile nonhemolytic reaction?

Infant or intrauterine transfusion

Washed RBCs are usually used in:

10-20%

In washed RBCs, about how much are lost in the process using saline?

25-35 GY

In irradiated RBCs, the recommended minimum dose of gamma irradiation is:

25 GY

In irradiated RBCs, the minimum radiation dose at the center of the unit is:

Original outdate or 28 days from irradiation, whichever comes first

What is the shelf life of irradiated RBCs?

6 days

In the preparation of frozen RBCs, the product should be frozen in glycerol within how many days of collection?

10 years

What is the shelf life of frozen RBCs?

24 hours

What is the shelf life of frozen RBCs after deglycerolization?

-65°C or -20°C

What is the storage temp for frozen RBCs?

Platelet concentrate / Random donor platelet

Which component is needed if the patient’s platelet count is less than 20,000/uL or if the pre-operation platelet count is less than 50,000/uL?

20-24 °C

Storage temperature of platelet concentrate / random donor platelet

4 hours

What is the shelf life of platelet concentrate after pooling?

5 days from collection with constant agitation

What is the shelf life of platelet concentrate right after collection?

≥ 5.5 x 10^10 platelets

1 unit of platelet concentrate contains:

≥ 3.0 x 10^11

1 unit of plateletpheresis unit or single donor platelet unit contains:

Fewer donors

Plateletpheresis unit or single donor platelet (SDP) exposes the recipient to:

350/uL or <500/uL

Granulocyte component is used for platelets with absolute granulocyte count of:

Granulocyte

Which component is used for patients with severe neutropenia with infection non-responsive to antibiotic therapy?

Septic infants

Granulocyte component is limited to:

20-24°C without agitation

What is the storage temp for Granulocyte component?

24 hours

What is the shelf life of granulocyte component?

> 1.0x x 10^10

1 unit of granulocyte contains:

8 hours of collection

In the preparation of FFP, plasma should be separated from whole blood within:

30-37°C for 30-45 mins

Fresh frozen plasma should be thawed in the water bath at:

Fresh frozen plasma

What is the component used for bleeding patients who require factors II, V, VII, IX, X?

Warfarin

Fresh frozen plasma reverses the effect of:

• TTP and HUS

• Patients w/ liver disease to prevent or correct bleeding

FFP is used for the treatment of:

-18°C for 1 year

Storage temp of FFP

1-6°C for 24 hours

Storage temp of FFP after thawing

All of the coagulation factors

Fresh frozen plasma contains:

Cryoprecipitate

Which component is prepared by thawing FFP at 1°-6°C, removing plasma, and refreezing within 1 hour?

• Fibrinogen = 150-220 mg

• AHF VIII:c = 80-120 IU

• vWf = 40-70%

• Factor XIII = 20-30%

• Fibronectin

Contents of cryoprecipitate:

Stored at room temp and transfused immediately; or transfuse within 6 hours

Thawed cryoprecipitate should be:

• Fibrinogen deficiency

• Hemophilia A

• Von Willebrand’s disease

• Factor XIII deficiency

• Used also as fibrin sealant

Cryoprecipitate is used for the treatment of:

TTP

Cryoprecipitate is NOT indicated for:

-18°C (1 year)

What is the storage temperature of cryoprecipitate?

Room temp (6 hours)

What is the storage temperature of cryoprecipitate after thawing?

Factor VIII concentrate or recombinant factor preparations are not available

Cryoprecipitate is used for treating hemophilia A and von Willebrand’s disease only if:

5 days

In the preparation of plasma derivatives, plasma may be separated from whole blood at any time during the unit’s shelf life for up to how many days after the expiration?

• Pasteurization

• Nanofiltration

• Solvent detergent

Different methods to ensure sterility of plasma derivatives include:

5 years when stored between 1-6 °C

What is the shelf life of plasma derivatives?

Plasma expanders

These are products that are transfused in patients suffering from hypovolemia or indicated among burn and shock patients

96% albumin + 4% globulin

What are the contents of Normal Serum Albumin (NSA)?

83% albumin + 17% globulin

What are the contents of Plasma Protein Fraction (PPF)?

• Ringer’s Lactate (Na, Cl, K, Ca, lactate ions)

• Normal Saline Solution (0.85-0.90% NaCl)

Crystalloid plasma expanders:

• Dextran (6-10%)

• Hydroxyethyl starch (HES)

Colloid plasma expanders:

4 hours (or 2-4 hours)

The administration of blood or its component intravenously is done within:

240 mL

At a rate of 60 drops per minute, how much blood can be transfused in an hour?

30 minutes after removing from the refrigerator

Infusion of whole blood/pRBC should start within:

Immediately

Platelet concentrates should be infused:

Immediately after thawing

Fresh frozen plasma should be infused:

Filtration

Removal of only plasma through a membrane; for therapeutic purposes

Adsorption

Removal of only a selected constituent of plasma with reinfusion of plasma after constituent is removed

48 hours (not more than 24 times a year)

Donation of platelets can be done every:

4 weeks

Donation of plasma can be done every:

Not more than twice a week, not more than 24 times a year

Leukocytes can be donated: