beams electrons transmitted through biological sample
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Scanning electron microsopy
Secondary electrons are emmited that are detected creating the image of a three dimensional surface
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TEM vs SEM
SEM is better at showing 3 demnesional surfaces more detail
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Standard fluroescence microscopy
fluorescent molecules label a particular type of cellular protein or organelle and absorbs light at particular wavelength and meits light at longer wavelenths
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Organelle
membrane bound compartment with its own unique structure and function
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Nucleus
compartment that is enclused by double-membrane structure called a nuclear envolope
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Lysosome
degreads macromolecules
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Chromatin
complex of protein and DNA inside the nucleus
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Nucleolus
Site for ribosome submunit assembley
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Nuclear pore
passageway for molecules into and out fo the nucleus
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Ribosome
site of polypeptide synthesis
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Plasma membrane
Membrane that controls movemnt of substances into and out of the cell, site of cell signaling
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Cytosol
site of many metabolic pathways (vast empty part of the cell)
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Centrosome
Site where microtubles grow and centrioles are found
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Rough ER
Site of protein and sorting and secrtion
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Smooth ER
Site of detoxification and lipid sythesis
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Mitochondrion
Site of ATP synthesis
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Cytoskeleton
Protien filaments that provide shape and aid in movement
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Peroxisome
Site where hydrogen peroxide and other harmful molecules are broken down
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Golgi apparatus
Site of modification, sorting, and secrtion of lipids and proteins (fedex)
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direct intercellular signaling
signals pass through a cell from the cytosol of one cell to adjacent cells
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Desmosomes
anchor cells to other cells
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Extracellular matrix
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Hemidesmosomes
anchor cells to the ECM
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Tight junctions
spaces between cells with “airtight seals”
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Gap junctions
direct connections between different cells
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Plasmodesmata
microscopic channels inbetween different cells, can open and close