Veterinary Hematology and Fluid Calculation Lecture Notes

Flashcards covering veterinary hematology vocabulary, blood cell count correction formulas, RBC indices, reticulocyte calculations, and fluid loss volume estimations.

WBC

White Blood Cell

RBC

Red Blood Cell

Plt

Platelet

PCV

Packed Cell Volume

Hb

Hemoglobin

MCV

Mean Cell Volume; defined as the average volume each RBC contains.

MCH

Mean Cell Hemoglobin; defined as the average amount of hemoglobin in each RBC.

MCHC

Mean Cell Hemoglobin Concentration; defined as the average concentration of Hemoglobin in each RBC.

T.P.

Total Protein

nRBC

Nucleated RBC's; these are similar in size to WBC's and may be mistaken for them when counting on a hemacytometer.

Corrected WBC count formula

$$\text{corrected WBC count} = \frac{\text{WBC count} \times 100}{\text{nRBC} + 100}$$

Absolute Differential formula

$\text{\# of WBC type} = \text{\% of WBC type} \times \text{WBC count}$ (where the percentage is in decimal form).

Common Canine/Feline White Blood Cells

Neutrophils, Lymphocytes, Monocytes, and Eosinophils.

Observed Reticulocyte % formula

$$\frac{\text{\# Reticulocytes}}{1000} \times 100$$

Corrected Observed Reticulocyte % formula

$$\text{Observed Reticulocyte \%} \times \frac{\text{PCV of patient}}{\text{Average PCV of species}}$$

Absolute Reticulocyte Count formula

$$\frac{\text{\# Reticulocytes}}{1000} \times \text{RBC count}$$

MCV calculation formula

$\text{MCV} = \frac{\text{PCV} \times 10}{\text{RBC count } (10^{6})}$; the unit is femptoliters ($fl$).

MCH calculation formula

$\text{MCH} = \frac{\text{Hb} \times 10}{\text{RBC count } (10^{6})}$; the unit is picograms ($pg$).

MCHC calculation formula

$\text{MCHC} = \frac{\text{Hb} \times 100}{\text{PCV}}$; the unit is $g/dl$.

Fibrinogen

A protein in the blood involved in blood clotting, always reported in $mg/dl$.

Fibrinogen calculation formula

$$\text{Fibrinogen} = \text{T.P. (\#1)} - \text{T.P. (\#2)}$$

T.P.:Fibrinogen Ratio formula

$\text{T.P.:Fibrinogen} = \text{T.P. (\#1)} \times \text{Fibrinogen } (g/dl)$; reported as a ratio per 1 part Fibrinogen (e.g., $5:1$).

Veterinary Rule for Fluid Loss

$$1 \text{ gram of body weight} = 1 \text{ ml of } H_{2}O$$

mls lost formula (lbs)

$$\text{mls lost} = \text{\% dehydrated} \times \text{animals weight (lbs)} \times 454$$

Liters lost formula (horse/kgs)

$$\text{Liters lost} = \text{\% dehydrated} \times \text{animals weight (kgs)}$$