1. inside the rod are a lot of optic disks stacked on top of one another
2. a lot of proteins on the disks. one is rhodospin (on a cone the same protein is called photopsin), a multimeric protein with 7 discs, which contains a small molecule called retinal (11-cis retinal). When light hit, comes through pupil and hits the retinal, then it rods, some of the light his rhodospin (which contains the retina) and causes the retinal to change conformation from bent to straight conformation (11-trans retinal)
3. when retinal changes shape, rhodopsin changes shape (closely linked molecules). this begins cascade
4. there is a molecule called transducin made of 3 different parts - alpha, beta, gamma that is attached to the rhodopsin
5. when the rhodopsin changes shape, transducin breaks from the rhodospin, and alpha subunit binds to another disk protein called phosphodiesterase (PDE)
6. PDE takes cGMP and converts it to regular GMP [ So when light hits, lower concentration and increases concentration of GMP]
7. Lots of Na+ channels on the rods allow NA+ ions to come in
* cGMP bound to Na+ channel, keeps the channel open and hence "ON", as cGMP concentration decreases, Na+ channel closes and turns Off
* when Na+ channels become unbound of cGMP, less NA+ enters the cell, then cell hyperpolarization and turn off
8. next, bipolar cells (2 variants: on center and off center)
* when light hits rod, turned off -> on center bipolar cells active, off center bipolar cells inactive
* when on center bipolar cells turned on, this activates on center retinal ganglion cell, when sends signal to optic nerve to brain.
*when dark, rod is turned on-> on center bipolar cells inactive, off center bipolar cells active
*when off center bipolar cells turned on, this activates off center retinal ganglion cell, when sends signal to optic nerve to