CC-Lab Ammonia & Uric Acid

Produced in the catabolism or deamination of amino acids and by bacterial metabolism in the lumen of the intestine

AMMONIA

Ammonia is removed from the circulation and converted to _____ in the liver.

Urea

causes swelling in the liver and brain

Reye’s syndrome

often affects children and teenagers recovering from a viral infection, most commonly the flu or chickenpox

Reye’s syndrome

Give me (1) disease correlation of Ammonia.

Hepatic failure

Complicated by its low concentration, instability, and pervasive contamination

ANALYTICAL METHOD

Glutamate Dehydrogenase Assay

ANALYTICAL METHOD

Whole blood ammonia concentration increases rapidly following specimen collection because of?

in vitro amino acid deamination

Venous blood should be obtained without ______ and placed on __ immediately

trauma, ice

Specimen Anticoagulant for Ammonia?

Heparin and EDTA

In, Ammonia tests, samples should be centrifuged at ______ within ________ of collection and the plasma or serum removed

0°C to 4°C, 20 minutes

Should, hemolysis be avoided in Ammonia tests? (Y/N)

Yes.

Can patients smoke before Ammonia tests?

Refrain from smoking for several hours

Metabolic waste product (end-product) of purine metabolism

URIC ACID

Is Uric Acid filtered by the glomerulus? (Y/N)

Yes.

98-100% of Uric acid is reabsorbed by PCT, while small amounts are secreted by DCT. (Y/N)

Yes.

Uric Acid is present as what in the plasma?

Monosodium Urate

Uric Acid is relatively insoluble, at blood pH. (Y/N)

Yes.

When the uric acid concentrations become greater than 6.8 mg/dL, the plasma is?

Saturated

Based on the oxidation of UA in PFF, with subsequent reduction of phosphotungstic acid to tungsten blue

CARAWAY METHOD

In Caraway Method, this provides alkaline pH for color development

Sodium carbonate

Type of Uric acid method that lacks specificity.

CARAWAY METHOD

In uricase method, uricase catalyzes the oxidation of Uric acid to?

allantoin

Uricase method, measures the differential absorption of uric acid and allantoin at what specific range (nm)?

293 nm

These can cause negative interference in Uricase method

Hemoglobin and xanthine

This method measures the hydrogen peroxide produced as Uric Acid Is converted to allantoin.

COUPLED-ENZYMATIC

Uric acid is oxidized to allantoin by uricase

The generated hydrogen peroxide reacts with 4 aminophenazone/ESPT to quinoneimine

ENZYMATIC-COLORIMETRIC

What is the Specimen used for Uric Acid?

Heparinized plasma,Serum, or Urine

Is fasting required for Uric Acid tests? (Y/N)

No.

In Uric Acid tests, should you avoid lipernic and hemolyzed specimens? (Y/N)

Yes.

In Uric Acid test, this should be removed from cells ASAP to prevent dilution by intracellular contents

Serum

How many days to refrigerate as sample for Uric Acid tests?

May be refrigerated for 3-5 days