micro - appendix A and B

Media consistency - solid (concentration, usage, other)

• 1.5 - 2% concentration

• Isolation of individual

• Anaerobic environment

Media consistency - semisolid (concentration, usage, other)

• less than .5%

• Bacterial motility determination

• Microaerophilic bacteria

Media consistency - liquid (concentration, usage, other)

• no gelling agent

• Growing large number or preparing inoculum

• Definitive morphology and maintain viability for transporting

Classification of agar - nutrient/basal (media, characteristics, examples)

• Basic media w/o special supplements

• For non-fastidious organism

• Nutrient agar, tryptic soy agar, peptone broth

Classification of agar - enriched (media, characteristics, examples)

• supplemented with additional nutrient

• For more fastidious organism for growth

• BAP, CHOC, Brain heart infusion agar

Classification of agar - selective (media, characteristics, examples)

• favors particular organisms while inhibiting others

• Addition of antibiotics, dyes, chemicla, pH, temp.

• MAC, MTM, BCYE-PAC, PEA

Classification of agar - differential (media, examples)

• growth of multiple organisms but allow morphologically distinguishable colonies

• BAP, MAC, HEA, MSA

Classification of agar - transport (characteristics, examples)

• protect the viability of microorganisms between collection and culturing

• Cary-Blair, Amies (wound), Stuart

Other types of media (3)

• storage media

• Assay media

• Maintenance media

SBA (ingredient, grow/inhibit, differentiation, organisms)

• 5% sheep blood

• All except Haemophilus spp.

• N/A

• columbia agar may support Neisseria spp.

Horse blood (ingredient, grow/inhibit, differentiation, organisms)

• 5% horse blood, contains X factor (heme) and V factor (NAD)

• support growth of Haemophilus influenzae

• Better visualization for GNR

• All

Rabbit blood (ingredient, grow/inhibit, differentiation, organisms)

• 7% rabbit blood

• N/A

• Beta-hemolysis for Haemophilus and gardenerella vaginalis

• Used for Bartonella spp.

CDC Anaerobic blood (ingredient, grow/inhibit, differentiation, organisms)

• sheep blood, X factor, vitamin K

• Better for GPC

• N/A

• Good for fastidious anerobes

CHOC (ingredient, grow/inhibit, differentiation, organisms)

• heated SBA with X & V factor

• N/A

• N/A

• Haemophilus spp and Neisseria spp.

Bacteroids bile esculin (ingredient, grow/inhibit, differentiation, organisms)

• Bile salt and iron source

• Only Bile resistant grows

• Hydrolysis of esculin causes black ppt

• Bacteroides fragilis

LKV (ingredient, grow/inhibit, differentiation, organisms)

• Kanamycin, Vancomycin, vit K

• Inhibits all cocci and facultative GNR

• N/A

• anaerobic like Bacteroids and Prevotella

PEA (ingredient, grow/inhibit, organisms)

• sheep blood, phenyl ethyl alcohol

• All GPC grow and inhibit GN (aerobic)

• Obligate anaerobes GN (mostly anaerobes)

BHI (grow/inhibit, differentiation)

• Grows bacteria, yeast, fungi

• Usually isolates bacteria and fungus

BCYE (ingredient, grow/inhibit, differentiation, organisms)