Verification of Constructs and DNA Sequencing Techniques

This set of flashcards covers key vocabulary and concepts related to verification of constructs, DNA assembly, and sequencing techniques used in molecular biology.

NEBuilder HI-FI DNA assembly

A method used to join multiple DNA fragments into a single construct.

Mini-prep

A procedure to purify plasmid DNA from a bacterial culture.

Patch plates

Plates used to grow individual bacterial colonies by transferring a small amount of bacteria.

Lysis buffer

A solution that breaks down cell membranes to release DNA.

Neutralization buffer

A solution used to halt the lysis process and precipitate cellular debris.

Restriction enzymes (e.g., NotI, AflII)

Molecular scissors used to cut DNA at specific sequences.

Agarose gel electrophoresis

A technique used to separate DNA fragments by size.

Spectrophotometer

An instrument used to measure the concentration of nucleic acids.

NCBI BLAST

A bioinformatics tool to compare nucleotide sequences against a database.

ABI file

A file format for DNA sequencing data.

Expected digestion pattern

The anticipated result of a DNA digestion based on the presence of restriction sites.