DNA replication vs Transcription

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24 Terms

1
DNA replication

The process of copying the entire DNA molecule to make new, identical double stranded DNA molecules.

This occurs during cell division to make sure that each new cell has the same genetic information.

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2
Transcription
The process of synthesizing RNA from a DNA template, which takes place in the nucleus and is the first step in gene expression.
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3
DNA polymerase

An enzyme that synthesizes new DNA strands during DNA replication.

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4
S phase

The phase of the cell cycle where DNA replication occurs.

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5
Thymine

A nitrogenous base used in DNA replication that pairs with adenine.

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6
Primer RNA

Short RNA sequence required to initiate DNA replication.

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7
Semiconservative replication

A type of DNA replication in which each new DNA molecule contains one original strand and one new strand.

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8
Ligase

An enzyme that joins DNA fragments together during DNA replication.

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9
Primase

An enzyme that synthesizes RNA primers needed for DNA replication.

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10
Single-stranded binding protein

Proteins that bind to and stabilize single-stranded DNA during DNA replication.

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11
Lagging strand

The DNA strand that is synthesized discontinuously in short segments during DNA replication.

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12
Single strand of RNA

The result of transcription, forming a complementary strand to the DNA template.

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13
RNA polymerase

A required enzyme that synthesizes RNA from a DNA template during transcription.

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14
Promoter

A sequence needed to initiate the formation of the RNA initiation complex in transcription.

(no primer needed)

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15
Uracil

A nitrogenous base used in transcription and RNA, that pairs with adenine and replaces thymine found in DNA.

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16
G phases

The phases of the cell cycle (G1 and G2) where transcription occurs.

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17
Lacks proofreading activity

Refers to transcription where there is no mechanism to correct errors during RNA synthesis.

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18
TATA box

A promoter sequence crucial for the initiation of transcription.

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19
Nucleus (eukaryotes)

The location where both transcription and DNA replication take place in eukaryotic cells.

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20
Cytoplasm (prokaryotes)

The location where both transcription and DNA replication occur in prokaryotic cells.

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21
DNA template

The original DNA strand used for the synthesis of the new DNA or RNA strand.

used in transcription and DNA replication

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22
Helicase

An enzyme that unwinds the DNA double helix during DNA replication and transcription.

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23
5’ to 3’

The direction in which both DNA and RNA strands are synthesized, referring to the carbon numbers on the DNA sugar backbone.

the way the strand goes in both transcription and DNA replication

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24
Complementary base pairing

The principle that allows adenine to pair with thymine (or uracil in RNA) and cytosine to pair with guanine.

has this in both transcription and DNA replication

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