Immunofluorescence Microscopy (Chapt. 4)

Immunofluorescence Microscopy

A method for visualizing the localization of specific proteins within cells or tissues using fluorescently labeled antibodies

Antibody

A protein that is covalently attached to a fluorescent dye and used to stain cells or tissues for specific proteins

Permeabilized

The process of treating cells or tissues to allow the passage of substances into and out of the cell, facilitating the interaction of antibodies with intracellular targets

Mitochondria

Cell organelles responsible for energy production through cellular respiration

Lysosomes

Cell organelles containing digestive enzymes involved in breaking down waste materials and cellular debris

Deconvolution Fluorescence Microscopy

A method that uses calculated point-spread functions to remove out-of-focus fluorescence, resulting in improved image resolution and clarity